Dmitriy Gushchin

PhD

Calculated based on number of publications stored in Pure and citations from Scopus
1989 …2024

Research activity per year

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Personal profile

Research interests

Development of new genome editing tools in CRISPR/Cas system. Using modern toolbox for genome editing.
I have a general interest in developing new instruments for Genome editing. Along this interest I have designed and developed new variants of Cas12a (CRISPR typeV) nucleases with new PAM specificities. I have developed new method for marker-free selection with Ouabain for ABE base editors. Using a model for R858L mutation in EGFR gene, I have shown clinically relevant level of mutation correction on endogenous alleles of the gene. This data has been presented at CRISPER Congress, Novosibirsk. In addition, I have developed a new method of genome mutation detection and quantitation. It is currently a gold standard in genome editing as T7E1 assay or Cel1 assay.

Study of gene expression regulation in relation to chromatin organization.
I have long-standing interest in chromatin-gene expression regulation field. Together with my colleagues, I have elucidated mechanisms of nucleosome mobilization by ATP-dependent Mi-2 complex in relation to promoter activation. In addition, we have characterized multiple ISWI ATPase complexes from Xenopus laevis and have shown functional conservation of an ACF/CHRAC homolog. Some subunits of these complexes are known as oncogenes in Humans. These opens a new and potentially important avenue in cancer research. In addition, we have used zinc-finger baes artificial transcriptional factors for specific regulation of targeted genes, achieving single-gene specificity and high efficiency of regulation.

Elucidation of Interleukin signaling by Jak/STAT signaling pathway. 
The JAK/STAT pathway is a signalling cascade that links the activation of specific receptors to specific gene expression. We have shown, for the first time, involvement of Jak kinases in Interferon-a and Interferon-g signalling. In addition, we have demonstrated activation of JAK kinases and STAT proteins by interleukin-2 and interferon alpha, but not the T cell antigen receptor, in human T lymphocytes.  Later, we have shown that interleukin-7 can induce the activation of Jak1, Jak3 and STAT5 proteins in murine T cells. All that have elucidated Jak/STAT signaling involvement in multiple Interleukin and Interferon pathways. This data laid a foundation in study of many signaling pathways.

Using genome-engineering tools for cancer research.
One of my current interests is the use of genome-engineering tools for cancer research.
There are multiple avenues for use of CRISPR/Cas genome engineering in cancer research. One of them development of new cellular models for drug development in practically all main cancer types. We have focused on lung cancer as a model system and considerable progress has been achieved in screening some chemical libraries in non-small lung adenocarcinoma cancer. In addition, genome editing can be used in cancer therapy, especially in non-solid cancers. As a side avenue, I am interested in developing of such approaches.

Personal profile

2023 - present
Nazarbaev University School of Medicine
Assistant Professor
PREVIOUS POSITIONS
2019 – 2022 Senior Research Scientist, Life Science center
Moscow Institute of Physics and Technology, Russia
Generated a number of KO lines related to brest cancer research
2021 – January-November Research Engineer
Ecole Polytechnique, Laboratory BIOC, Palaiseau, France
Generated a number of KO lines and single amino acid substitutions, related to cell motility.
2015 – 2019 Research Fellow
Center for Genome Engineering
Institute of Basic Science, Republic of Korea
Developed Cpf1 (CRISPR typeV) proteins with new PAM specificities. Developed method for marker-free selection for ABE base editors.
2000 – 2014 Scientist I – III
Sangamo BioSciences, Richmond, CA, USA
Developed new method of genome mutation detection and quantitation. Demonstrated for the first time, targeted deletions by two Zinc-Finger nucleases. Obtained 10 USA patents. Co-author over 20 peered-reviewed publications including Nature, Nature Biotechnology.
1997 – 2000 Visiting Scientist, Laboratory of Molecular Embryology,
NICHD, NIH, Bethesda, MD, USA
Elucidated mechanisms of nucleosome mobilization by ATP-dependent Mi-2 complex, published in Science, Biochemistry.
1995 – 1997 Visiting Scientist, Human Genome Group,
CMBB, ANL, Argonne, IL, USA
Developed new approach for oliogonucleotide chip manufacturing and application in biome characterization. Patented and acquired by Motorola.
1991 – 1995 Research Fellow, Biochemical Regulatory Mechanisms Laboratory,
Imperial Cancer Research Fund, London, UK
Discovered involvement of Jak kinases in interferon signalling, published in Nature.
1990 – 1991 Research Scientist,Laboratory of Chromosome Structure
Institute of Molecular Biology, Moscow, Russia
Demonstrated histone tails involvement in maintaining chromatin structure during transcription, published in Cell.

Publications

  1. Publications in peer-reviewed scientific journals, peer-reviewed conference proceedings and/or monographs.

In summary, 47 publications cited 11095 times, with h-index 39, according to Google scholar1 (October 2019).

  • Zeitler, B., Froelich, S., Marlen, K., Shivak, D., Yu, Q., Li, D., Pearl, J., Miller, J., Zhang,L., Paschon, D.,Hinkley, S., Ankoudinova, I., Lam, S., Guschin, D.,  Kopan, L., Cherone, J., Nguyen, H., Qiao, G., Ataei, Y.,Mendel, M., Amora, R., Surosky, R., Laganiere, J., Vu, B.J., Narayanan, A., Sedaghat, Y., Tillack, K., Thiede,C., Gärtner, A., Kwak, S., Bard, J., Mrzljak, L., Park, L., Heikkinen, T., Lehtimäki, K., Svedberg, M.,Häggkvist, J., Tari, L., Tóth, M., Varrone, A., Halldin, C., Kudwa, A., Ramboz, S., Day, M., Kondapalli, J.,Surmeier, D.J., Urnov, F., Gregory, P., Rebar, E., Muñoz-Sanjuán, I., Zhang, H.S., Allele-selectivetranscriptional repression of mutant HTT for the  treatment of Huntington’s disease. Nature Medicine, 2019, on-line publication July1, doi: 10.1038/s41591-019-0478-3
  • Bulanova D, Ianevski A, Bugai A, Akimov Y, Kuivanen S, Paavilainen H, Kakkola L, Nandania J, Turunen L, Ohman T, Ala-Hongisto H, Pesonen HM, Kuisma MS, Honkimaa A, Walton EL, Oksenych V, Lorey MB, Guschin D, Shim J, Kim J, Than TT, Chang SY, Hukkanen V, Kulesskiy E, Marjomaki VS, Julkunen I, Nyman TA, Matikainen S, Saarela JS, Sane F, Hober D, Gabriel G, De Brabander JK, Martikainen M, Windisch MP, Min JY, Bruzzone R, Aittokallio T, Vähä-Koskela M, Vapalahti O, Pulk A, Velagapudi V, KainovDE. Antiviral Properties of Chemical Inhibitors of Cellular Anti-Apoptotic Bcl-2 Proteins. Viruses, 2017, Sep 25;9(10). pii: E271
  • Genovese P, Schiroli G, Conway A, Ferrari S, Guschin DY, Tomaso TD, Albano L, Marin V, Giuliani E, Gregory PD, Holmes MC, Gentner B, Bovolenta C, Cost GJ, Lombardo A, Naldini L. Towards Clinical Translation of Hematopoietic Stem Cell Gene Editing for the Correction of SCID-X1 Mutations. Mol Ther. 2016;24:S16
  • Beane JD, Lee G, Zheng Z, Mendel M, Abate-Daga D, Bharathan M, Black M, Gandhi N, Yu Z, Chandran S, Giedlin M, Ando D, Miller J, Paschon D, Guschin D, Rebar EJ, Reik A, Holmes MC, Gregory PD, Restifo NP, Rosenberg SA, Morgan RA, Feldman SA. Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma. Mol Ther. 2015; 23(8):1380-90. doi: 10.1038/ mt.2015.71./33 citations/.
  • Conway A, Guschin DY, Genovese P, Lombardo A, Naldini L, Gregory PD, Holmes MC, Cost GJ. Gene Correction of IL2RG in Human Hematopoietic Stem and Progenitor Cells. Mol Ther. 2015;23:S273
  • Miller JC, Zhang L, Xia DF, Campo JJ, Ankoudinova IV, Guschin DY, Babiarz JE, Meng X, Hinkley SJ, Lam SC, Paschon DE, Vincent AI, Dulay GP, Barlow KA, Shivak DA, Leung E, Kim JD, Amora R, Urnov FD, Gregory PD, Rebar EJ. Improved specificity of TALE-based genome editing using an expanded RVD repertoire. Nat Methods. 2015; 12(5):465-71. doi: 10.1038/nmeth.3330. /53 сitations/.
  • Crane AM, Kramer P, Bui JH, Chung WJ, Li XS, Gonzalez-Garay ML, Hawkins F, Liao W, Mora D, Choi S, Wang J, Sun HC, Paschon DE, Guschin DY, Gregory PD, Kotton DN, Holmes MC, Sorscher EJ, Davis BR. Targeted correction and restored function of the CFTR gene in cystic fibrosis induced pluripotent stem cells. Stem Cell Reports. 2015; 4(4):569-77. doi: 10.1016/j.stemcr.2015. 02.005. /69 сitations/.
  • Davis B, Crane AM, Kramer P, Bui JH, Chung WJ, Li XS, Gonzalez-Garay ML, Hawkins F, Liao W, Mora D, Choi S, Wang J, Sun HC, Paschon DE, Guschin DY, Gregory PD, Kotton DN, Holmes MC, Sorscher EJ. Targeted correction and restored function of the CFTR gene in cystic fibrosis induced pluripotent stem cells. Mol Ther. 2015;23:S74
  • Jiang J, Jing Y, Cost GJ, Chiang JC, Kolpa HJ, Cotton AM, Carone DM, Carone BR, Shivak DA, Guschin DY, Pearl JR, Rebar EJ, Byron M, Gregory PD, Brown CJ, Urnov FD, Hall LL, Lawrence JB. Translating dosage compensation to trisomy 21. Nature. 2013; 500(7462):296-300. doi: 10.1038/nature12394. /207 сitations/.
  • Reik, Chang K., Stehling-Sun S., Zhou Y., Lee G. K., Truong L., Wood T., Zhang Z, Luong A, Chan A, Liu P, Miller J. C, Paschon D. E, Guschin D. Y, Zhang L, Yannaki E, Giedlin M. A, Rebar E. J, Gregory, P. D, Urnov FD, Papayannopoulou T, Stamatoyannopoulos G. Targeted Gene Modification In Hematopoietic Stem Cells: A Potential Treatment For Thalassemia and Sickle Cell Anemia. Blood, 2013; 122(21), 434.
  • Llewellyn N, Miller JC, Campo J, Exline C, Mulhern O, Henley J, Wang J, Guschin D, Xia D, Ankouidinova I, Gregory PD, Zhang L, Rebar EJ, Holmes MC, Cannon PM. Next Generation TALENs Mediate Efficient Disruption of the CCR5 Gene in Human HSCs. Mol Ther. 2013;21:S72
  • Bui JH, Crane AM, Li XS, Kramer P, Gonzalez-Garay M ,Liao W, Wang J, Sun HC, Paschon DE, Guschin DY, Gregory PD, Kotton DN, HolmesMC, Davis BR. Allele-Preferred Targeted Correction of CFTR Gene in Cystic Fibrosis Induced Pluripotent Stem Cells. Mol Ther. 2013;21:S44
  • Zhang HS, Liu D, Huang Y, Schmidt S, Hickey R, Guschin D, Su H, Jovin IS, Kunis M, Hinkley S, Liang Y, Hinh L, Spratt SK, Case CC, Rebar EJ, Ehrlich B, Gregory PD, Giordano FJ. A Designed Zinc-finger Transcriptional Repressor of Phospholamban Improves Function of the Failing Heart. Mol Ther. 2012; 20(8):1508-1515. doi: 10.1038/mt.2012.80. /18 сitations/.
  • Soldner F, Laganière J, Cheng AW, Hockemeyer D, Gao Q, Alagappan R, Khurana V, Golbe LI, Myers RH, Lindquist S, Zhang L, Guschin D, Fong LK, Vu BJ, Meng X, Urnov FD, Rebar EJ, Gregory PD, Zhang HS, Jaenisch R. Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell. 2011; 146(2):318-31. doi: 10.1016/j.cell.2011.06. 019. /614 сitations/.
  • Young JJ, Cherone JM, Doyon Y, Ankoudinova I, Faraji FM, Lee AH, Ngo C, Guschin DY, Paschon DE, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Harland RM, Zeitler B. Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A. 2011; 108(17):7052-7. doi: 10.1073/pnas.1102030108. /124 сitations/.
  • Laganiere J, Kells AP, Lai JT, Guschin D, Paschon DE, Meng X, Fong LK, Yu Q, Rebar EJ, Gregory PD, Bankiewicz KS, Forsayeth J, Zhang HS. An engineered zinc finger protein activator of the endogenous glial cell line-derived neurotrophic factor gene provides functional neuroprotection in a rat model of Parkinson's disease. J Neurosci. 2010;30(49):16469-74. doi: 10.1523/JNEUROSCI.2440-10.2010. /57 сitations/.
  • Guschin DY, Waite AJ, Katibah GE, Miller JC, Holmes MC, Rebar EJ. A rapid and general assay for monitoring endogenous gene modification. Methods Mol Biol. 2010; 649:247-56. doi: 10.1007/978-1-60761-753-2_15. /332 сitations/.
  • Liu PQ, Chan EM, Cost GJ, Zhang L, Wang J, Miller JC, Guschin DY, Reik A, Holmes MC, Mott JE, Collingwood TN, Gregory PD. Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases. Biotechnol Bioeng. 2010; 106(1):97-105. doi: 10.1002/bit.22654. /113 сitations/.
  • Perez EE, Wang J, Miller JC, Jouvenot Y, Kim KA, Liu O, Wang N, Lee G, Bartsevich VV, Lee YL, Guschin DY, Rupniewski I, Waite AJ, Carpenito C, Carroll RG, Orange JS, Urnov FD, Rebar EJ, Ando D, Gregory PD, Riley JL, Holmes MC, June CH. Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol. 2008; 26(7):808-16. doi: 10.1038/nbt1410. /954 сitations/.
  • Johnston LC, Su X, Maguire-Zeiss K, Horovitz K, Ankoudinova I, Guschin D, Hadaczek P, Federoff HJ, Bankiewicz K, Forsayeth J. Human interleukin-10 gene transfer is protective in a rat model of Parkinson's disease. Mol Ther. 2008; 16(8):1392-9. doi: 10.1038/mt.2008.113. /70 сitations/.
  • Santiago Y, Chan E, Liu PQ, Orlando S, Zhang L, Urnov FD, Holmes MC, Guschin D, Waite A, Miller JC, Rebar EJ, Gregory PD, Klug A, Collingwood TN. Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A. 2008; 105(15):5809-14. doi: 10.1073/pnas. 0800940105. /366 citations/.
  • Yokoi K, Zhang HS, Kachi S, Balaggan KS, Yu Q, Guschin D, Kunis M, Surosky R, Africa LM, Bainbridge JW, Spratt SK, Gregory PD, Ali RR, Campochiaro PA. Gene transfer of an engineered zinc finger protein enhances the anti-angiogenic defense system. Mol Ther. 2007; 15(11):1917-23. /24 сitations/.
  • Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ. An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol. 2007; 25(7):778-85. /910 citations/.
  • Tan S, Guschin D, Davalos A, Lee YL, Snowden AW, Jouvenot Y, Zhang HS, Howes K, McNamara AR, Lai A, Ullman C, Reynolds L, Moore M, Isalan M, Berg LP, Campos B, Qi H, Spratt SK, Case CC, Pabo CO, Campisi J, Gregory PD. Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity. Proc Natl Acad Sci U S A. 2003; 100(21):11997-2002. /162 citations/.
  • Urnov FD, Wolffe AP, Guschin D. Molecular mechanisms of corepressor function. Curr Top Microbiol Immunol. 2001; 254:1-33. /37 сitations/.
  • Kikyo N, Wade PA, Guschin D, Ge H, Wolffe AP. Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI. Science. 2000; 289 (5488):2360-2. /242 citations/./253 сitations/.
  • Wolffe AP, Urnov FD, Guschin D. Co-repressor complexes and remodelling chromatin for repression. Biochem Soc Trans. 2000; 28(4):379-86. /77 сitations/.
  • Guschin D, Geiman TM, Kikyo N, Tremethick DJ, Wolffe AP, Wade PA. Multiple ISWI ATPase complexes from xenopus laevis. Functional conservation of an ACF/CHRAC homolog. J Biol Chem. 2000; 275(45):35248-55. /82 сitations/.
  • Guschin D, Wade PA, Kikyo N, Wolffe AP. ATP-Dependent histone octamer mobilization and histone deacetylation mediated by the Mi-2 chromatin remodeling complex. Biochemistry. 2000; 39(18):5238-45. /96 сitations/.
  • Wolffe AP, Guschin D. Review: chromatin structural features and targets that regulate transcription. J Struct Biol. 2000; 129(2-3):102-22. /371 сitations/.
  • Guschin D, Wolffe AP. SWItched-on mobility. Curr Biol. 1999; 9(19): R742-6. /28 сitations/.
  • Chandler SP, Guschin D, Landsberger N, Wolffe AP. The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA. Biochemistry. 1999; 38(22):7008-18. /183 сitations/.
  • Guschin D, Chandler S, Wolffe AP. Asymmetric linker histone association directs the asymmetric rearrangement of core histone interactions in a positioned nucleosome containing a thyroid hormone response element. Biochemistry. 1998; 37(24):8629-36. /35 сitations/.
  • Wong J, Patterton D, Imhof A, Guschin D, Shi YB, Wolffe AP. Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase. EMBO J. 1998; 17(2):520-34. /179 сitations/.
  • Guschin D, Yershov G, Zaslavsky A, Gemmell A, Shick V, Proudnikov D, Arenkov P, Mirzabekov A. Manual manufacturing of oligonucleotide, DNA, and protein microchips. Anal Biochem. 1997; 250(2):203-11. /380 сitations/.
  • Guschin DY, Mobarry BK, Proudnikov D, Stahl DA, Rittmann BE, Mirzabekov AD. Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology. Appl Environ Microbiol. 1997; 63(6): 2397-402. /456 сitations/.
  • Kohlhuber F, Rogers NC, Watling D, Feng J, Guschin D, Briscoe J, Witthuhn BA, Kotenko SV, Pestka S, Stark GR, Ihle JN, Kerr IM. A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses. Mol Cell Biol. 1997; 17(2):695-706. /224 сitations/.
  • Yershov G, Barsky V, Belgovskiy A, Kirillov E, Kreindlin E, Ivanov I, Parinov S, Guschin D, Drobishev A, Dubiley S, Mirzabekov A. DNA analysis and diagnostics on oligonucleotide microchips. Proc Natl Acad Sci U S A. 1996; 93 (10):4913-8. /660 citations/.
  • Briscoe J, Guschin D, Rogers NC, Watling D, Müller M, Horn F, Heinrich P, Stark GR, Kerr IM. JAKs, STATs and signal transduction in response to the interferons and other cytokines. Philos Trans R Soc Lond B Biol Sci. 1996; 351(1336):167-71. /76 сitations/.
  • Foxwell BM, Beadling C, Guschin D, Kerr I, Cantrell D. Interleukin-7 can induce the activation of Jak 1, Jak 3 and STAT 5 proteins in murine T cells. Eur J Immunol. 1995; 25(11):3041-6.
  • Guschin D, Rogers N, Briscoe J, Witthuhn B, Watling D, Horn F, Pellegrini S, Yasukawa K, Heinrich P, Stark GR, Kerr I. A major role for the protein tyrosine kinase JAK1 in the JAK/STAT signal transduction pathway in response to interleukin-6. EMBO J. 1995; 14(7):1421-9. /424 сitations/.
  • Beadling C, Guschin D, Witthuhn BA, Ziemiecki A, Ihle JN, Kerr IM, Cantrell DA. Activation of JAK kinases and STAT proteins by interleukin-2 and interferon alpha, but not the T cell antigen receptor, in human T lymphocytes. EMBO J. 1994; 13(23):5605-15. /221 сitations/.
  • Briscoe J, Guschin D, Müller M. Signal transduction. Just another signalling pathway. Curr Biol. 1994; 4(11):1033-5. /66 сitations/.
  • Müller M, Briscoe J, Laxton C, Guschin D, Ziemiecki A, Silvennoinen O, Harpur AG, Barbieri G, Witthuhn BA, Schindler C, Pellegrini S, Wilks AF, Ihle JN, Stark GR, Kerr I. The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction. Nature. 1993; 366(6451):129-35. /737 citations/.
  • Watling D, Guschin D, Müller M, Silvennoinen O, Witthuhn BA, Quelle FW, Rogers NC, Schindler C, Stark GR, Ihle JN, Kerr I. Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway. Nature. 1993; 366(6451):166-70. /531 сitations/.
  • Gushchin DY, Ebralidse KK, Mirzabekov AD. Structure of nucleosomes. Localization of the H2A and H2B histone segments interacting with DNA using DNA-protein crosslinking. Biol. (Moscow). 1991; 25(5):1400-1411/19 сitations/.
  • Nacheva GA, Guschin DY, Preobrazhenskaya OV, Karpov VL, Ebralidse KK, Mirzabekov AD. Change in the pattern of histone binding to DNA upon transcriptional activation. Cell. 1989; 58(1):27-36. /270 сitations/.

 

1 http://scholar.google.com/citations?user=bLKWhxYAAAAJ&hl=en&oi=ao

  1. Research monographs, chapters
    • Guschin D.Y. Artificial nucleases containing zinc finger domains. History of creation and application in fundamental research and practical medicine. Editing genes and genomes. Ans. Ed. C. M. Zakian. Novosibirsk, 2016. Publishing house of the SB RAS.
    • Guschin D.Y. A rapid and general assay for detecting and quantifying endogenous gene modification. Editing genes and genomes. Ans. Ed. C. M. Zakian. Novosibirsk, 2018. Publishing house of the SB RAS.

Intellectual Property

  1. Granted patents.
    • Guschin D, Urnov F. Targeted deletion of cellular DNA sequences. 2017; US 9695442 B2.
    • Cost GJ, Gregory PD, Guschin D, Holmes MC, Miller JC, Paschon D, Rebar EJ, Reik A, Urnov F, Zhang L. Methods and compositions for treatment of a genetic condition. 2017; US 9650648 B2.
    • Shukla V, Gupta M, Urnov F, Guschin D, De Both MJ, Bundock P, Sastry-Dent L. Targeted modification of malate dehydrogenase. 2016; US 9523098 B2.
    • Ainley WM, Webb SR, Samuel P, Guschin DY, Miller JC, Zhang L.Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks. 2016; US 9493779 B2.
    • Cost GJ, Guschin DY, Urnov F. Targeted genomic modification with partially single-stranded donor molecules. 2016; US 9255259 B2.
    • Guschin DY, Holmes MC, Paschon D, Tam P. Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (CFTR) gene. US 9161995 B2
    • Cost GJ, Guschin DY, Urnov F. Targeted genomic modification with partially single-stranded donor molecules. 2015; US 9005973 B2.
    • Guschin D, Holmes M. Targeted deletion of cellular DNA sequences. 2013; US 8409861 B2.
    • Wolffe A,Urnov F, Guschin D, Collingwood T, Li XY, Johnstone B.Pharmacogenomics and identification of drug targets by reconstruction of signal transduction pathways based on sequences of accessible regions. 2003; US 6610489 B2.
    • Wolffe A,Urnov F, Guschin D, Collingwood T, Li XY, Johnstone B. Methods for designing exogenous regulatory molecules. 2003; US 6511808 B2.
    • Mirzabekov AD, Guschin DY, Chick V, Drobyshev A, Fotin A, Yershov G, Lysov Y. Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable. 2002; US 6458584 B1.
    • Mirzabekov AD, Yershov GM, Guschin DY, Gemmel AM, Chick VV, Proudnikov DY, Timofeev EN. Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction. 2002; US 6465174 B1.

Education/Academic qualification

Molecular biology, PhD, Study of chromatin by chemical DNA-protein cross-links, RAS - Engelhardt Institute of Molecular Biology

Sept 1 1986Apr 20 1990

Award Date: Aug 4 1990

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    Loboda, A. P., Adonin, L. S., Zvereva, S. D., Guschin, D. Y., Korneenko, T. V., Telegina, A. V., Kondratieva, O. K., Frolova, S. E., Pestov, N. B. & Barlev, N. A., Mar 2023, In: International journal of molecular sciences. 24, 5, 4982.

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  • Antiviral properties of chemical inhibitors of cellular anti-apoptotic Bcl-2 proteins

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  • Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma

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  • Improved specificity of TALE-based genome editing using an expanded RVD repertoire

    Miller, J. C., Zhang, L., Xia, D. F., Campo, J. J., Ankoudinova, I. V., Guschin, D. Y., Babiarz, J. E., Meng, X., Hinkley, S. J., Lam, S. C., Paschon, D. E., Vincent, A. I., Dulay, G. P., Barlow, K. A., Shivak, D. A., Leung, E., Kim, J. D., Amora, R., Urnov, F. D., Gregory, P. D., & 1 othersRebar, E. J., Apr 29 2015, In: Nature Methods. 12, 5, p. 465-471 7 p.

    Research output: Contribution to journalArticlepeer-review

    70 Citations (Scopus)