Projects per year
Personal profile
Research interests
Research
Functional responsibilities of cathepsin G (CatG) in immunity, including bivalent regulation of major histocompatibility complex class I (MHC) molecules, which underscore a novel role of CatG within the immune system. Special Interests: Immune evasion of tumor cells and pathogens, modulating of the proteolytic activity of CatG, functional properties of CatG in T cells, immunosenescence.
Our model of how exogenous CatG modulates cell surface expression of MHC I.
CatG proteolytically cleaves the N-terminal end of the extracellular domain of protease-activated receptor 1 (PAR1). As a result, the tethered activation ligand flips to the extracellular loop 2 and recruits intracellular G protein, which is capable of downstream signal transduction.
However, cleavage of the extracellular part of PAR1 by CatG can also lead to receptor inactivation, so-called dis-arming; thereby the tethered ligand as well as three extracellular loops of PAR1 are digested and blocks G protein-mediated signaling. This can be further enhanced by the interaction of lactoferrin (LF) with CatG. LF increases the proteolytic activity of CatG and augments a CatG-mediated upregulation of MHC I molecules on the cell surface. The MHC I recycling pathway, for instance, is important for loading a new set of antigenic peptides (viral derived or tumor-associated) to MHC I molecules in order to be displayed on the cell surface for CD8+ T cell inspection. Distinct viruses or tumor cells prevent the synthesis of nascent MHC I molecules or interfere with the MHC I recycling pathway. The advantage of CatG-mediated upregulation of cell surface MHC I molecules is the efficacy to reuse MHC I within the MHC I recycling pathway, where MHC I molecules are pushed out to the cell surface instead of being degraded in the lysosome, as an additional model regarding how CatG circumvents immune evasion of viruses or tumor cells.
Lectures
Spring 2020:
BIOL 341 Biochemistry I and Recitation
BIOL 341 Biochemistry Laboratory
BIOL 410 Introduction to Immunology
Fall 2019:
BIOL 341 Biochemistry I and Recitation
BIOL 410 Introduction to Immunology
Spring 2019:
BIOL 341 Biochemistry I and Recitation
BIOL 341 Biochemistry Laboratory
BIOL 480 Molecular Immunology
Fall 2018:
BIOL 410 Introduction to Immunology
BIOL 623 Advanced Immunology
Spring 2018:
BIOL 341 Biochemistry I and Recitation
External positions
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Projects
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Microencapsulated cells as a platform for sustained delivery of therapeutic antibodies
6/1/17 → 7/31/21
Project: Monitored by Research Administration
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Development of a diagnostic test for the detection of human antibodies to SARS-CoV-2 (COVID-19)
Hortelano, G., Schoenbach, C., Ramankulov, Y., Burster, T. & Poddighe, D.
Project: Monitored by Research Administration
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Battling host proteases to prevent productive infection by SARS-CoV-2 and severe complications of COVID-19
Project: Monitored by Research Administration
Research output
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Activity-Based Probes to Utilize the Proteolytic Activity of Cathepsin G in Biological Samples
Burster, T., Gärtner, F., Knippschild, U. & Zhanapiya, A., 2021, In: Frontiers in Chemistry. 9, p. 628295Research output: Contribution to journal › Review article › peer-review
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Neutrophil Elastase and Proteinase 3 Cleavage Sites Are Adjacent to the Polybasic Sequence within the Proteolytic Sensitive Activation Loop of the SARS-CoV-2 Spike Protein
Mustafa, Z., Zhanapiya, A., Kalbacher, H. & Burster, T., Mar 16 2021, In: ACS Omega. 6, 10, p. 7181-7185 5 p.Research output: Contribution to journal › Article › peer-review
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Regulation of mhc i molecules in glioblastoma cells and the sensitizing of nk cells
Burster, T., Gärtner, F., Bulach, C., Zhanapiya, A., Gihring, A. & Knippschild, U., Mar 2021, In: Pharmaceuticals. 14, 3, 236.Research output: Contribution to journal › Review article › peer-review
Open Access -
Application of an Activity-Based Probe to Determine Proteolytic Activity of Cell Surface Cathepsin G by Mass Cytometry Data Acquisition
Gärtner, F., Knippschild, U. & Burster, T., 2020, (Accepted/In press) In: ACS Omega. 5, 43, p. 28233-28238 6 p.Research output: Contribution to journal › Article › peer-review
Open Access2 Citations (Scopus) -
Application of a novel FAM-conjugated activity-based probe to determine cathepsin G activity intracellularly
Schroeder, R., Grzywa, R., Wirtz, C. R., Sienczyk, M. & Burster, T., Jan 1 2020, In: Analytical Biochemistry. 588, 113488.Research output: Contribution to journal › Article › peer-review
4 Citations (Scopus)