Projects per year
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Research interests
Research
Functional responsibilities of cathepsin G (CatG) in immunity, including bivalent regulation of major histocompatibility complex class I (MHC) molecules, which underscore a novel role of CatG within the immune system. Special Interests: Immune evasion of tumor cells and pathogens, modulating of the proteolytic activity of CatG, functional properties of CatG in T cells, immunosenescence.
Our model of how exogenous CatG modulates cell surface expression of MHC I.
CatG proteolytically cleaves the N-terminal end of the extracellular domain of protease-activated receptor 1 (PAR1). As a result, the tethered activation ligand flips to the extracellular loop 2 and recruits intracellular G protein, which is capable of downstream signal transduction.
However, cleavage of the extracellular part of PAR1 by CatG can also lead to receptor inactivation, so-called dis-arming; thereby the tethered ligand as well as three extracellular loops of PAR1 are digested and blocks G protein-mediated signaling. This can be further enhanced by the interaction of lactoferrin (LF) with CatG. LF increases the proteolytic activity of CatG and augments a CatG-mediated upregulation of MHC I molecules on the cell surface. The MHC I recycling pathway, for instance, is important for loading a new set of antigenic peptides (viral derived or tumor-associated) to MHC I molecules in order to be displayed on the cell surface for CD8+ T cell inspection. Distinct viruses or tumor cells prevent the synthesis of nascent MHC I molecules or interfere with the MHC I recycling pathway. The advantage of CatG-mediated upregulation of cell surface MHC I molecules is the efficacy to reuse MHC I within the MHC I recycling pathway, where MHC I molecules are pushed out to the cell surface instead of being degraded in the lysosome, as an additional model regarding how CatG circumvents immune evasion of viruses or tumor cells.
Lectures
Spring 2020:
BIOL 341 Biochemistry I and Recitation
BIOL 341 Biochemistry Laboratory
BIOL 410 Introduction to Immunology
Fall 2019:
BIOL 341 Biochemistry I and Recitation
BIOL 410 Introduction to Immunology
Spring 2019:
BIOL 341 Biochemistry I and Recitation
BIOL 341 Biochemistry Laboratory
BIOL 480 Molecular Immunology
Fall 2018:
BIOL 410 Introduction to Immunology
BIOL 623 Advanced Immunology
Spring 2018:
BIOL 341 Biochemistry I and Recitation
External positions
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Projects
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Development of a diagnostic test for the detection of human antibodies to SARS-CoV-2 (COVID-19)
Hortelano, G., Schoenbach, C., Ramankulov, Y., Burster, T. & Poddighe, D.
Project: Monitored by Research Administration
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Battling host proteases to prevent productive infection by SARS-CoV-2 and severe complications of COVID-19
Project: Monitored by Research Administration
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Microencapsulated cells as a platform for sustained delivery of therapeutic antibodies
6/1/17 → 12/31/20
Project: Monitored by Research Administration
Research Output
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Application of an Activity-Based Probe to Determine Proteolytic Activity of Cell Surface Cathepsin G by Mass Cytometry Data Acquisition
Gärtner, F., Knippschild, U. & Burster, T., 2020, (Accepted/In press) In: ACS Omega. 5, 43, p. 28233-28238 6 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Application of a novel FAM-conjugated activity-based probe to determine cathepsin G activity intracellularly
Schroeder, R., Grzywa, R., Wirtz, C. R., Sienczyk, M. & Burster, T., Jan 1 2020, In: Analytical Biochemistry. 588, 113488.Research output: Contribution to journal › Article › peer-review
3 Citations (Scopus) -
Cathepsin G and its Dichotomous Role in Modulating Levels of MHC Class I Molecules
Burster, T., Knippschild, U., Molnár, F. & Zhanapiya, A., Aug 1 2020, In: Archivum Immunologiae et Therapiae Experimentalis. 68, 4, 25.Research output: Contribution to journal › Review article › peer-review
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Considering the Experimental use of Temozolomide in Glioblastoma Research
Herbener, V. J., Burster, T., Goreth, A., Pruss, M., von Bandemer, H., Baisch, T., Fitzel, R., Siegelin, M. D., Karpel-Massler, G., Debatin, K-M., Westhoff, M-A. & Strobel, H., Jun 4 2020, In: Biomedicines. 8, 6Research output: Contribution to journal › Review article › peer-review
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Effects of yolkin on the immune response of mice and its plausible mechanism of action
Obmińska-Mrukowicz, B., Szczypka, M., Lis, M., Pawlak, A., Suszko-Pawłowska, A., Sysak, A., Zambrowicz, A., Burster, T., Kocięba, M., Artym, J., Zaczyńska, E., Kochanowska, I. & Zimecki, M., Apr 2020, In: Immunology Letters. 220, p. 21-31 11 p.Research output: Contribution to journal › Article › peer-review