• 863 Citations
  • 17 h-Index
20022020
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Personal profile

Research interests

Research

Functional responsibilities of cathepsin G (CatG) in immunity, including bivalent regulation of major histocompatibility complex class I (MHC) molecules, which underscore a novel role of CatG within the immune system. Special Interests: Immune evasion of tumor cells and pathogens, modulating of the proteolytic activity of CatG, functional properties of CatG in T cells, immunosenescence. 

Our model of how exogenous CatG modulates cell surface expression of MHC I.

CatG proteolytically cleaves the N-terminal end of the extracellular domain of protease-activated receptor 1 (PAR1). As a result, the tethered activation ligand flips to the extracellular loop 2 and recruits intracellular G protein, which is capable of downstream signal transduction.

However, cleavage of the extracellular part of PAR1 by CatG can also lead to receptor inactivation, so-called dis-arming; thereby the tethered ligand as well as three extracellular loops of PAR1 are digested and blocks G protein-mediated signaling. This can be further enhanced by the interaction of lactoferrin (LF) with CatG. LF increases the proteolytic activity of CatG and augments a CatG-mediated upregulation of MHC I molecules on the cell surface. The MHC I recycling pathway, for instance, is important for loading a new set of antigenic peptides (viral derived or tumor-associated) to MHC I molecules in order to be displayed on the cell surface for CD8+ T cell inspection. Distinct viruses or tumor cells prevent the synthesis of nascent MHC I molecules or interfere with the MHC I recycling pathway. The advantage of CatG-mediated upregulation of cell surface MHC I molecules is the efficacy to reuse MHC I within the MHC I recycling pathway, where MHC I molecules are pushed out to the cell surface instead of being degraded in the lysosome, as an additional model regarding how CatG circumvents immune evasion of viruses or tumor cells.

Lectures

Spring 2020:

BIOL 341 Biochemistry I and Recitation

BIOL 341 Biochemistry Laboratory

BIOL 410 Introduction to Immunology

Fall 2019:

BIOL 341 Biochemistry I and Recitation

BIOL 410 Introduction to Immunology

Spring 2019:

BIOL 341 Biochemistry I and Recitation

BIOL 341 Biochemistry Laboratory

BIOL 480 Molecular Immunology 

Fall 2018:

BIOL 410 Introduction to Immunology

BIOL 623 Advanced Immunology

Spring 2018:

BIOL 341 Biochemistry I and Recitation

External positions

Fingerprint Dive into the research topics where Timo Burster is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

Cathepsin G Medicine & Life Sciences
Dendritic Cells Medicine & Life Sciences
Cathepsins Medicine & Life Sciences
Peptide Hydrolases Medicine & Life Sciences
Antigen Presentation Medicine & Life Sciences
cathepsin S Medicine & Life Sciences
Major Histocompatibility Complex Medicine & Life Sciences
Peptides Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Projects 2017 2020

Antibodies
Monoclonal Antibodies
Therapeutics
Health Care Costs
Antineoplastic Agents

Research Output 2002 2020

  • 863 Citations
  • 17 h-Index
  • 47 Article
  • 7 Review article
  • 6 Conference contribution

Application of a novel FAM-conjugated activity-based probe to determine cathepsin G activity intracellularly

Schroeder, R., Grzywa, R., Wirtz, C. R., Sienczyk, M. & Burster, T., Jan 1 2020, In : Analytical Biochemistry. 588, 113488.

Research output: Contribution to journalArticle

Cathepsin G
Avidin
Organophosphonates
T-cells
Flow cytometry

Effects of yolkin on the immune response of mice and its plausible mechanism of action

Obmińska-Mrukowicz, B., Szczypka, M., Lis, M., Pawlak, A., Suszko-Pawłowska, A., Sysak, A., Zambrowicz, A., Burster, T., Kocięba, M., Artym, J., Zaczyńska, E., Kochanowska, I. & Zimecki, M., Apr 2020, In : Immunology Letters. 220, p. 21-31 11 p.

Research output: Contribution to journalArticle

Jurkat Cells
Thymocytes
Humoral Immunity
Bone Marrow Cells
Proteins
1 Citation (Scopus)

Cell surface cathepsin G can be used as an additional marker to distinguish T cell subsets

Penczek, A. & Burster, T., Apr 2019, In : Biomedical Reports. 10, 4, p. 245-249 5 p.

Research output: Contribution to journalArticle

Cathepsin G
T-cells
T-Lymphocyte Subsets
Regulatory T-Lymphocytes
T-Lymphocytes

FAM-conjugated MARS116 can be applied to detect intracellular cathepsin G activity”

Burster, T., 2019, 25th Polish Peptide Symposium, Wojanow, Poland. Invited keynote speaker.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cathepsin G
Organophosphonates
Avidin
Flow Cytometry
T-Lymphocytes

Immune Cells and Immunosenescence

Bischof, J., Gärtner, F., Zeiser, K., Kunz, R., Schreiner, C., Hoffer, E., Burster, T., Knippschild, U. & Zimecki, M., Jan 1 2019, In : Folia Biologica. 65, 2, p. 53-63 11 p.

Research output: Contribution to journalArticle

Aptitude
Immune system
Immune System
Aging of materials
Pathogens

Prizes

Humboldt Award, Poland

Timo Burster (Recipient), 2013

Prize

immunology
therapy