Activities per year
This project is a continuation of active collaboration with Scientists at the Institute of Microbiology and Virology (IMV) in Almaty Kazakhstan that has been ongoing for over ten years. In 2006, the PI was one of the collaborators on a grant funded for 3 years by the ISTC (International Science and Technology Center, on a project with the IMV entitled “The surveillance of new antibiotics from Kazakhstan soils actinomycetes, active against methicillin-resistant Staphylococci with multiple drug-resistances”. This project which was a collaboration with Dr. Trenozhnikova Lyudmila at the IMV, was successfully completed and described in two publications in 2012 and 2013. This continued project funded by NU focuses on the characterization of novel antibiotics isolated from Actinomycetes obtained from extreme environments of Kazakhstan that are active against drug-resistant hospital-associated pathogens as described in the proposal.
Overview of the overall proposed Project: three Specific Aims
Having characterized the producer organisms and extracts with known antagonistic activities against gram positive and gram negative pathogens, and with the antibiotic extracts produced and in storage, we are in a good position to expand on this study. We propose in this project application, three Specific Aims (SA’s). The first two SA’s (SA#1 and SA#2) are designed to further characterize the extracts; this is a series of effort to move forward the identification of active components within the extracts. For the third SA (SA#3), we propose to return to the field to further isolate and identify other extremophile strains that produce potent and novel antimicrobial or anticancer agents, using our established methods of sampling and culturing. These producer strains will be characterized further as in SA2#1 and #2, to identify antimicrobial or anticancer compounds that are novel, which could then be further developed for therapeutic applications and commercialization.
We plan to continue preparing extracts from extremophilic actinomycetes here at Nazarbayev University (NU), and perform all the related tests in the NUSOM laboratory. The extracts that have already shown antagonistic effect against pathogens and had differential peaks on HPLC will be prepared again to prepare more of the extracts for future testings and chemical analysis. Those extract will be fractionated using Bioassay-guided fractionation and each fraction will be tested for the inhibitory activity. This way it will be possible to separate the active component and identify it, using other chemical methods, such as mass spectrometry and NMR.
There is an increasing rate of deaths due to antimicrobial resistant (AMR) pathogens. This is the justification for our project which focuses on discovering novel antibiotic that would be effective against drug resistant hospital pathogens, which is the aim of our project. There has not been a research conducted on screening extremophilic actinomycetes under different growth conditions, since it can affect production of different, uncharacterized antibiotics. Students from different schools (SST) and departments within NUSOM have been involved in this project during summer internships, and as a part of the master’s thesis project. They have performed extraction, inhibitory activity tests, including disc diffusion assay and wells assay, and chemical tests, in collaboration with IMV and the RDC.
|Effective start/end date||10/1/16 → 12/31/19|
Preliminary study to screen extremophile microorganisms and plant extracts from unique ecosystems of Kazakhstan as potential producers of novel antiviral agents
Azliyati Azizan (Invited speaker)2017
Activity: Participating in event › Participation in conference
(1) NIH (National Institute of Health), USA, (2) Infectious Diseases, AIDS and Clinical Immunology Research Center (IDACIRC), Tbilisi, Georgia, and (3) International Science and Technology Center (ISTC), Astana, Kazakhstan
Azliyati Azizan (Invited speaker)Apr 30 2017 → May 4 2017
Activity: Participating in event › Participation in workshop, seminar
Azliyati Azizan (Speaker)Jun 21 2017
Activity: Talk or presentation › Invited talk