The purpose of the study. Investigation of the polymorphism of genes of superoxide dismutase (SOD) and amylase in cultivated wheat species and wild relatives, the possibility of using them as molecular genetic markers to assess the genetic diversity of wheat varieties. Methods. The objects of research were wheat varieties cultivated in different periods in Kazakhstan, distant relatives and wild wheat species. The material was kindly provided by the staff of the laboratory of the gene pool of the SPC ZH im. A.I. Baraev, and also obtained from the USDA genetic resource collections (http://wheat.pw.usda.gov/ GG3), John Innes Center (Germplasm Resources Unit, BBSRC) (http://data.jic.bbsrc.ac.uk/cgi-bin/germplasm/cereals.asp), All-Russian Institute of Plant Industry named after N.I. Vavilov (VIR) (http://220.127.116.11/virdb). Isolation of total DNA from etiolated seedlings was performed using the CTAB method. Selection of primers for PCR amplification, in silico PCR, analysis of oligonucleotides, and multiple alignment of DNA sequences were performed using the FastPCR program (http:// primerdigital.com/fastpcr.html). Bioinformatic analysis of candidate genes was carried out using the NCBI database (http:// www.ncbi.nlm.nih.gov/gene), UniProt http://www.uniprot.org/uniprot), Ensembl Plants (http://plants.ensembl.org). Results. Analysis of genetic diversity based on the spectra of superoxide dismutase and amylase isoenzymes in cultivated wheat species and wild relatives cultivated in different breeding periods showed that the level of differentiation between the studied groups was quite high, most of the genetic diversity was revealed within the groups. The genetic diversity of modern wheat varieties has a lower number of polymorphic loci and effective alleles of storage proteins, SOD, and amylases genes in comparison with distant relatives and ancient varieties from the world collection. Practical significance. The study of the polymorphism of the gene families of superoxide dismutase and amylase will increase the efficiency of identification of varieties and hybrids, study of their heterogeneity and targeted selection of parental pairs for crosses.
|Agrarian Bulletin of the Urals
|Published - Dec 1 2020