Three-dimensional imaging of morphology, structural and functional property distributions in the volume of various soft matter systems has been obtained by scanning probe microscopy (SPM) tomography with nanometer resolution. We have performed SPM tomography with a specially designed instrument, which combines SPM with anultra-microtome. The successive sectioning by the microtome knife and subsequent imaging of the remaining sample block face surface by common variety of SPM modes allows for 3D reconstruction of organisation and different material properties with z-resolution down to ~10 nm and xy resolution of ~1 nm or better. In this chapter we introduce some approaches for SPM tomography and discuss calibration procedures, data acquisition algorithms and volume visualisation routines. We will present some volume reconstruction results from several types of soft matter systems such as bacteria membranes, biomedical implants and few functional polymer systems, and critically discuss limitations and future prospects of SPM tomography by microtoming.
|Title of host publication||3D Reconstruction|
|Subtitle of host publication||Methods, Applications and Challenges|
|Publisher||Nova Science Publishers, Inc.|
|Number of pages||16|
|Publication status||Published - Jan 1 2013|
ASJC Scopus subject areas
- Computer Science(all)