A cell kinetic analysis of human umbilical vein endothelial cells

Lena Kalashnik, Collette J. Bridgeman, Andrea R. King, Sheila E. Francis, Sergey Mikhalovsky, Corrin Wallis, Stephen P. Denyer, David Crossman, R. G A Faragher

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cultures of normal human cells 'age' and become senescent in vitro due to a continuously declining mitotic fraction. Although endothelial cells represent a tissue of major relevance in the development of age-related vascular disease, the rate at which these cells senesce has never been systematically measured in culture. Accordingly the population kinetics of human vascular endothelial cells (HUVECs) serially passaged in vitro has been studied in order to determine (i) the rate of decline in the growth fraction; (ii) the rate of increase of the senescent fraction and (iii) the relationship between changes in these parameters and the baseline rate of apoptosis. Immunocytochemical visualisation of the growth fraction using antisera to the proliferation marker pKi67 showed a rate of decline in the growth fraction of 4.43±0.31% per population doubling. This was not accompanied by any change in cell cycle time as assessed using time lapse video microscopy. The number of senescent cells within the population increased at a rate of 6.47±0.3% as assessed by senescence associated β-galactosidase activity. The baseline rate of apoptosis as measured by TUNEL remained essentially unchanged (0.31±0.07%) during this process. These data show (i) that senescence and apoptosis are unrelated processes in HUVEC and (ii) that senescent cells rapidly and progressively accumulate in dividing populations of endothelial cells. The physiological relevance of these observations is discussed. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalMechanisms of Ageing and Development
Volume120
Issue number1-3
DOIs
Publication statusPublished - Dec 1 2000
Externally publishedYes

Fingerprint

Endothelial cells
Human Umbilical Vein Endothelial Cells
Endothelial Cells
Kinetics
Apoptosis
Cell culture
Population
Growth
Cells
Galactosidases
Video Microscopy
In Situ Nick-End Labeling
Vascular Diseases
Immune Sera
Cell Cycle
Microscopic examination
Visualization
Cell Count
Tissue
In Vitro Techniques

Keywords

  • Apoptosis
  • Human cells
  • Kinetic

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Kalashnik, L., Bridgeman, C. J., King, A. R., Francis, S. E., Mikhalovsky, S., Wallis, C., ... Faragher, R. G. A. (2000). A cell kinetic analysis of human umbilical vein endothelial cells. Mechanisms of Ageing and Development, 120(1-3), 23-32. https://doi.org/10.1016/S0047-6374(00)00179-2

A cell kinetic analysis of human umbilical vein endothelial cells. / Kalashnik, Lena; Bridgeman, Collette J.; King, Andrea R.; Francis, Sheila E.; Mikhalovsky, Sergey; Wallis, Corrin; Denyer, Stephen P.; Crossman, David; Faragher, R. G A.

In: Mechanisms of Ageing and Development, Vol. 120, No. 1-3, 01.12.2000, p. 23-32.

Research output: Contribution to journalArticle

Kalashnik, L, Bridgeman, CJ, King, AR, Francis, SE, Mikhalovsky, S, Wallis, C, Denyer, SP, Crossman, D & Faragher, RGA 2000, 'A cell kinetic analysis of human umbilical vein endothelial cells', Mechanisms of Ageing and Development, vol. 120, no. 1-3, pp. 23-32. https://doi.org/10.1016/S0047-6374(00)00179-2
Kalashnik L, Bridgeman CJ, King AR, Francis SE, Mikhalovsky S, Wallis C et al. A cell kinetic analysis of human umbilical vein endothelial cells. Mechanisms of Ageing and Development. 2000 Dec 1;120(1-3):23-32. https://doi.org/10.1016/S0047-6374(00)00179-2
Kalashnik, Lena ; Bridgeman, Collette J. ; King, Andrea R. ; Francis, Sheila E. ; Mikhalovsky, Sergey ; Wallis, Corrin ; Denyer, Stephen P. ; Crossman, David ; Faragher, R. G A. / A cell kinetic analysis of human umbilical vein endothelial cells. In: Mechanisms of Ageing and Development. 2000 ; Vol. 120, No. 1-3. pp. 23-32.
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