A controlled release system for simultaneous delivery of three human perivascular stem cell-derived factors for tissue repair and regeneration

Nurlan Mansurov, William C W Chen, Hassan Awada, Johnny Huard, Yadong Wang, Arman Saparov

Research output: Contribution to journalArticle

Abstract

Transplanted stem/progenitor cells improve tissue healing and regeneration anatomically and functionally, mostly due to their secreted trophic factors. However, harsh conditions at the site of injury, including hypoxia, oxidative and inflammatory stress, increased fibrosis and insufficient angiogenesis, and in some cases immunological response or incompatibility, are detrimental to stem cell survival. To overcome the complexity and deficiencies of stem cell therapy, the coacervate delivery platform is deemed promising because it offers controlled and sustained release using heparin to recapitulate the binding and stabilization of extracellular proteins by heparan sulphates in native tissues. Here we show that recombinant alternatives of three key factors [vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6)], commonly produced by perivascular stem cells under various stress conditions, can be successfully incorporated into a heparin-based coacervate. We characterized the release profile of the triply incorporated factors from the complex coacervate. The coacervate-released factors were able to exert their desired biological activities in vitro: VEGF stimulated human umbilical vein endothelial cell proliferation, MCP-1 elevated macrophage migration and IL-6 increased IgM production by IL-6-dependent cell line. Thus, a controlled release system can be used for simultaneous delivery of three stem cell-derived factors and could be useful for tissue repair and regenerative medicine.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
DOIs
Publication statusE-pub ahead of print - May 8 2017
Externally publishedYes

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Stem Cell Factor
Thromboplastin
Stem cells
Regeneration
Repair
Stem Cells
Tissue
Interleukin-6
Chemokine CCL2
Proteins
Vascular Endothelial Growth Factor A
Heparin
Regenerative Medicine
Heparitin Sulfate
Macrophages
Endothelial cells
Human Umbilical Vein Endothelial Cells
Cell proliferation
Cell- and Tissue-Based Therapy
Bioactivity

Keywords

  • Journal Article

Cite this

A controlled release system for simultaneous delivery of three human perivascular stem cell-derived factors for tissue repair and regeneration. / Mansurov, Nurlan; Chen, William C W; Awada, Hassan; Huard, Johnny; Wang, Yadong; Saparov, Arman.

In: Journal of Tissue Engineering and Regenerative Medicine, 08.05.2017.

Research output: Contribution to journalArticle

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