A highly allergenic fragment of the major timothy grass pollen allergen, Phl p 5, defined by a human monoclonal IgE antibody

S. Flicker, S. Vrtala, P. Steinberger, L. Vangelista, D. Kraft, Rudolf Valenta

Research output: Contribution to journalArticle

Abstract

We report the characterization of a domain of the major timothy grass pollen allergen, Phl p 5A, which contains the binding site for a human monoclonal IgE antibody. The human monoclonal IgE antibody fragment (Fab) was obtained from an IgE combinatorial library constructed from lymphocytes of a grass pollen-allergic patient. An 11.2-kD N-terminal fragment representing approximately one third of the complete Phl p 5A allergen could be identified to contain the binding site for the IgE Fab. The rPhl p 5A fragment revealed an extremely high allergenic activity in skin test experiments which in some cases equaled that of the complete Phl p 5A allergen. The rPhl p 5A domain thus represents an allergen fragment containing several IgE epitopes in a configuration optimal for efficient effector cell activation. We suggest the rPhl p 5A fragment and the corresponding IgE Fab as paradigmatic tools to explore the structural requirements for highly efficient effector cell activation.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalInternational Archives of Allergy and Immunology
Volume124
Issue number1-3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Phleum
Pollen
Allergens
Immunoglobulin E
Monoclonal Antibodies
Binding Sites
Immunoglobulin Fragments
Poaceae
Skin Tests
Epitopes
Lymphocytes

Keywords

  • Allergen
  • Epitope
  • Monoclonal human IgE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

A highly allergenic fragment of the major timothy grass pollen allergen, Phl p 5, defined by a human monoclonal IgE antibody. / Flicker, S.; Vrtala, S.; Steinberger, P.; Vangelista, L.; Kraft, D.; Valenta, Rudolf.

In: International Archives of Allergy and Immunology, Vol. 124, No. 1-3, 2001, p. 80-84.

Research output: Contribution to journalArticle

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AU - Valenta, Rudolf

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