Abstract
The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.
| Original language | English |
|---|---|
| Pages (from-to) | 99-103 |
| Number of pages | 5 |
| Journal | Cytogenetic and Genome Research |
| Volume | 66 |
| Issue number | 2 |
| Publication status | Published - 1994 |
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Keywords
- 3T3 Cells
- Animals
- Antibodies, Monoclonal
- Blotting, Western
- Chromosomal Proteins, Non-Histone
- Drosophila
- HeLa Cells
- Heterochromatin
- Humans
- In Situ Hybridization, Fluorescence
- Interphase
- Metaphase
- Mice
- Journal Article
- Research Support, Non-U.S. Gov't
Cite this
A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin. / Wreggett, K A; Hill, F; James, P S; Hutchings, A; Butcher, G W; Singh, P B.
In: Cytogenetic and Genome Research, Vol. 66, No. 2, 1994, p. 99-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin
AU - Wreggett, K A
AU - Hill, F
AU - James, P S
AU - Hutchings, A
AU - Butcher, G W
AU - Singh, P B
PY - 1994
Y1 - 1994
N2 - The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.
AB - The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.
KW - 3T3 Cells
KW - Animals
KW - Antibodies, Monoclonal
KW - Blotting, Western
KW - Chromosomal Proteins, Non-Histone
KW - Drosophila
KW - HeLa Cells
KW - Heterochromatin
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - Interphase
KW - Metaphase
KW - Mice
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Article
C2 - 8287692
VL - 66
SP - 99
EP - 103
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 2
ER -