A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin

K A Wreggett, F Hill, P S James, A Hutchings, G W Butcher, P B Singh

Research output: Contribution to journalArticle

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Abstract

The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalCytogenetic and Genome Research
Volume66
Issue number2
Publication statusPublished - 1994

Fingerprint

Heterochromatin
Monoclonal Antibodies
Proteins
Centromere
Conserved Sequence
Interphase
Human Chromosomes
Product Packaging
Metaphase
Genes
Chromatin
DNA
Drosophila heterochromatin protein 1

Keywords

  • 3T3 Cells
  • Animals
  • Antibodies, Monoclonal
  • Blotting, Western
  • Chromosomal Proteins, Non-Histone
  • Drosophila
  • HeLa Cells
  • Heterochromatin
  • Humans
  • In Situ Hybridization, Fluorescence
  • Interphase
  • Metaphase
  • Mice
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin. / Wreggett, K A; Hill, F; James, P S; Hutchings, A; Butcher, G W; Singh, P B.

In: Cytogenetic and Genome Research, Vol. 66, No. 2, 1994, p. 99-103.

Research output: Contribution to journalArticle

Wreggett, K A ; Hill, F ; James, P S ; Hutchings, A ; Butcher, G W ; Singh, P B. / A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin. In: Cytogenetic and Genome Research. 1994 ; Vol. 66, No. 2. pp. 99-103.
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AU - Butcher, G W

AU - Singh, P B

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KW - Animals

KW - Antibodies, Monoclonal

KW - Blotting, Western

KW - Chromosomal Proteins, Non-Histone

KW - Drosophila

KW - HeLa Cells

KW - Heterochromatin

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Interphase

KW - Metaphase

KW - Mice

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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