A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin

K A Wreggett; F Hill; P S James; A Hutchings; G W Butcher; P B Singh

A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin

K A Wreggett, F Hill, P S James, A Hutchings, G W Butcher, P B Singh

Department of Medicine

Research output: Contribution to journal › Article › peer-review

175 Citations (Scopus)

Abstract

The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.

Original language	English
Pages (from-to)	99-103
Number of pages	5
Journal	Cytogenetic and Genome Research
Volume	66
Issue number	2
Publication status	Published - 1994

Keywords

3T3 Cells
Animals
Antibodies, Monoclonal
Blotting, Western
Chromosomal Proteins, Non-Histone
Drosophila
HeLa Cells
Heterochromatin
Humans
In Situ Hybridization, Fluorescence
Interphase
Metaphase
Mice
Journal Article
Research Support, Non-U.S. Gov't

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@article{1729c8b3d61e4795a04a9956cec77fd7,

title = "A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin",

abstract = "The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.",

keywords = "3T3 Cells, Animals, Antibodies, Monoclonal, Blotting, Western, Chromosomal Proteins, Non-Histone, Drosophila, HeLa Cells, Heterochromatin, Humans, In Situ Hybridization, Fluorescence, Interphase, Metaphase, Mice, Journal Article, Research Support, Non-U.S. Gov't",

author = "Wreggett, {K A} and F Hill and James, {P S} and A Hutchings and Butcher, {G W} and Singh, {P B}",

year = "1994",

language = "English",

volume = "66",

pages = "99--103",

journal = "Cytogenetic and Genome Research",

issn = "1424-8581",

publisher = "S. Karger AG",

number = "2",

}

TY - JOUR

T1 - A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin

AU - Wreggett, K A

AU - Hill, F

AU - James, P S

AU - Hutchings, A

AU - Butcher, G W

AU - Singh, P B

PY - 1994

Y1 - 1994

N2 - The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.

AB - The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.

KW - 3T3 Cells

KW - Animals

KW - Antibodies, Monoclonal

KW - Blotting, Western

KW - Chromosomal Proteins, Non-Histone

KW - Drosophila

KW - HeLa Cells

KW - Heterochromatin

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Interphase

KW - Metaphase

KW - Mice

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

C2 - 8287692

VL - 66

SP - 99

EP - 103

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 2

ER -