A minimal receptor-Ig chimera of human FcεRI α-chain efficiently binds secretory and membrane IgE

Luca Vangelista, Michela Cesco-Gaspere, Roberto Lorenzi, Oscar Burrone

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We constructed a soluble minimal receptor-Ig chimera in which the two extracellular domains of human FcεRI α-chain (D1 and D2) were fused to the dimerizing C-terminal domain of human IgG1 heavy chain (γ1-CH3). The protein was expressed and actively secreted by Chinese hamster ovary (CHO) cells as a fully glycosylated soluble dimeric protein. It showed efficient binding both to human membrane-bound IgE isoforms and to the two secretory IgE isoforms. Moreover, the dimeric receptor binds IgE with the expected 1:2 stoichiometry. The receptor-Ig chimera, in 2-fold molar excess, inhibited engagement of secretory IgE to rat basophilic leukemia cells expressing the human αβγ receptor. Full self-nature and inability to bind Fcγ receptors make this protein an attractive candidate for clinical applications and a novel biotechnological tool for atopic allergy research.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalProtein Engineering
Volume15
Issue number1
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Immunoglobulin E
Proteins
Membranes
Protein Isoforms
Cells
Allergies
IgE Receptors
Fc Receptors
Stoichiometry
Rats
Immunoglobulin G
Cricetulus
Ovary
Hypersensitivity
Leukemia
Research

Keywords

  • Binding
  • Chimera
  • Dimerization
  • FcεRI
  • IgE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

A minimal receptor-Ig chimera of human FcεRI α-chain efficiently binds secretory and membrane IgE. / Vangelista, Luca; Cesco-Gaspere, Michela; Lorenzi, Roberto; Burrone, Oscar.

In: Protein Engineering, Vol. 15, No. 1, 2002, p. 51-57.

Research output: Contribution to journalArticle

Vangelista, Luca ; Cesco-Gaspere, Michela ; Lorenzi, Roberto ; Burrone, Oscar. / A minimal receptor-Ig chimera of human FcεRI α-chain efficiently binds secretory and membrane IgE. In: Protein Engineering. 2002 ; Vol. 15, No. 1. pp. 51-57.
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