A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells

J Kampinga, F G Kroese, G H Pol, P Nieuwenhuis, F Haag, P B Singh, B Roser, R Aspinall

Research output: Contribution to journalArticle

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Abstract

A hybridoma producing the monoclonal antibody HIS42 was isolated from a fusion between spleen cells from a BALB/c mouse immunized with rat thymocytes and the fusion partner SP2/0. This antibody recognizes a minor subset of T cells in every haplotype of rat tested so far. The subpopulation of HIS42 positive T cells contains both CD4+ and CD8+ cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS42 induces T cell proliferation in the presence of interleukin-2. In contrast to lymph node T cells, a number of thymocytes were found to express HIS42 only in the cytoplasm or together with membrane expression. Most bright HIS42 surface labelled thymocytes were also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS-PAGE analyses of the membrane molecules immunoprecipitated by HIS42 show two bands on unreduced gels. One of these bands (85 kd) runs as two separate bands at 35 and 48 kd on reduction. The other much weaker broad band (approximately 100 kd) is hardly affected by reduced conditions. Taken together these data suggest that HIS42 is directed against a determinant on the rat T cell receptor for antigen, which is common to a small number of T cells.

Original languageEnglish
Pages (from-to)289-95
Number of pages7
JournalInternational Immunology
Volume1
Issue number3
Publication statusPublished - 1989

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T-Lymphocyte Subsets
T-Cell Antigen Receptor
Thymocytes
Monoclonal Antibodies
T-Lymphocytes
Membranes
Hybridomas
Sepharose
Haplotypes
Interleukin-2
Polyacrylamide Gel Electrophoresis
Cytoplasm
Spleen
Cell Count
Lymph Nodes
Gels
Cell Proliferation
Antibodies
Population

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Cytoplasm
  • Epitopes
  • Lymphocyte Activation
  • Lymphoid Tissue
  • Rats
  • Rats, Inbred Strains
  • Receptors, Antigen, T-Cell
  • Species Specificity
  • T-Lymphocyte Subsets
  • Tissue Distribution
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Kampinga, J., Kroese, F. G., Pol, G. H., Nieuwenhuis, P., Haag, F., Singh, P. B., ... Aspinall, R. (1989). A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells. International Immunology, 1(3), 289-95.

A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells. / Kampinga, J; Kroese, F G; Pol, G H; Nieuwenhuis, P; Haag, F; Singh, P B; Roser, B; Aspinall, R.

In: International Immunology, Vol. 1, No. 3, 1989, p. 289-95.

Research output: Contribution to journalArticle

Kampinga, J, Kroese, FG, Pol, GH, Nieuwenhuis, P, Haag, F, Singh, PB, Roser, B & Aspinall, R 1989, 'A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells', International Immunology, vol. 1, no. 3, pp. 289-95.
Kampinga, J ; Kroese, F G ; Pol, G H ; Nieuwenhuis, P ; Haag, F ; Singh, P B ; Roser, B ; Aspinall, R. / A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells. In: International Immunology. 1989 ; Vol. 1, No. 3. pp. 289-95.
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AU - Kampinga, J

AU - Kroese, F G

AU - Pol, G H

AU - Nieuwenhuis, P

AU - Haag, F

AU - Singh, P B

AU - Roser, B

AU - Aspinall, R

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N2 - A hybridoma producing the monoclonal antibody HIS42 was isolated from a fusion between spleen cells from a BALB/c mouse immunized with rat thymocytes and the fusion partner SP2/0. This antibody recognizes a minor subset of T cells in every haplotype of rat tested so far. The subpopulation of HIS42 positive T cells contains both CD4+ and CD8+ cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS42 induces T cell proliferation in the presence of interleukin-2. In contrast to lymph node T cells, a number of thymocytes were found to express HIS42 only in the cytoplasm or together with membrane expression. Most bright HIS42 surface labelled thymocytes were also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS-PAGE analyses of the membrane molecules immunoprecipitated by HIS42 show two bands on unreduced gels. One of these bands (85 kd) runs as two separate bands at 35 and 48 kd on reduction. The other much weaker broad band (approximately 100 kd) is hardly affected by reduced conditions. Taken together these data suggest that HIS42 is directed against a determinant on the rat T cell receptor for antigen, which is common to a small number of T cells.

AB - A hybridoma producing the monoclonal antibody HIS42 was isolated from a fusion between spleen cells from a BALB/c mouse immunized with rat thymocytes and the fusion partner SP2/0. This antibody recognizes a minor subset of T cells in every haplotype of rat tested so far. The subpopulation of HIS42 positive T cells contains both CD4+ and CD8+ cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS42 induces T cell proliferation in the presence of interleukin-2. In contrast to lymph node T cells, a number of thymocytes were found to express HIS42 only in the cytoplasm or together with membrane expression. Most bright HIS42 surface labelled thymocytes were also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS-PAGE analyses of the membrane molecules immunoprecipitated by HIS42 show two bands on unreduced gels. One of these bands (85 kd) runs as two separate bands at 35 and 48 kd on reduction. The other much weaker broad band (approximately 100 kd) is hardly affected by reduced conditions. Taken together these data suggest that HIS42 is directed against a determinant on the rat T cell receptor for antigen, which is common to a small number of T cells.

KW - Animals

KW - Antibodies, Monoclonal

KW - Cytoplasm

KW - Epitopes

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KW - Lymphoid Tissue

KW - Rats

KW - Rats, Inbred Strains

KW - Receptors, Antigen, T-Cell

KW - Species Specificity

KW - T-Lymphocyte Subsets

KW - Tissue Distribution

KW - Journal Article

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