A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope

G. R. Newman, B. Jasani, E. D. Williams

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde-picric acid mixture, and embedded in 'L R White', a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate-lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.

Original languageEnglish
Pages (from-to)543-555
Number of pages13
JournalThe Histochemical Journal
Volume15
Issue number6
DOIs
Publication statusPublished - Jun 1983
Externally publishedYes

Fingerprint

Electrons
Antigens
Plastics
Fixatives
Haptens
Palatine Tonsil
Glutaral
Immersion
Dehydration
Citric Acid
Pancreas
Perfusion
Insulin
Staining and Labeling
Lead
picric acid
uranyl acetate

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. / Newman, G. R.; Jasani, B.; Williams, E. D.

In: The Histochemical Journal, Vol. 15, No. 6, 06.1983, p. 543-555.

Research output: Contribution to journalArticle

Newman, G. R. ; Jasani, B. ; Williams, E. D. / A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. In: The Histochemical Journal. 1983 ; Vol. 15, No. 6. pp. 543-555.
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