Abstract
A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde-picric acid mixture, and embedded in 'L R White', a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate-lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.
| Original language | English |
|---|---|
| Pages (from-to) | 543-555 |
| Number of pages | 13 |
| Journal | The Histochemical Journal |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Anatomy
- Cell Biology
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A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. / Newman, G. R.; Jasani, B.; Williams, E. D.
In: The Histochemical Journal, Vol. 15, No. 6, 06.1983, p. 543-555.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope
AU - Newman, G. R.
AU - Jasani, B.
AU - Williams, E. D.
PY - 1983/6
Y1 - 1983/6
N2 - A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde-picric acid mixture, and embedded in 'L R White', a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate-lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.
AB - A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde-picric acid mixture, and embedded in 'L R White', a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate-lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020964608&partnerID=8YFLogxK
U2 - 10.1007/BF01954145
DO - 10.1007/BF01954145
M3 - Article
VL - 15
SP - 543
EP - 555
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 6
ER -