A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope

G. R. Newman, B. Jasani, E. D. Williams

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde-picric acid mixture, and embedded in 'L R White', a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate-lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.

Original languageEnglish
Pages (from-to)543-555
Number of pages13
JournalThe Histochemical Journal
Volume15
Issue number6
DOIs
Publication statusPublished - Jun 1 1983

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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