Abnormal expression and function of the E-cadherin-catenin complex in gastric carcinoma cell lines

A. U. Jawhari, M. Noda, M. J.G. Farthing, M. Pignatelli

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44 Citations (Scopus)

Abstract

Dysfunction of the cadherin-catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, α, β and γ-catenin and p120(ctn), and of the adenomatous polyposis coil protein (APC), together with function of the cadherin-catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, α, β and γ-catenin, p120(ctn) and APC. Abnormalities of E-cadherin, α- and β-catenin expression, were associated with disturbance of E-cadherin-catenin complex composition, loss of membranous localization and loss of calcium-dependent aggregation in six gastric carcinoma cell lines, APC protein expression and interaction with β-catenin was preserved in five cell lines. We demonstrate frequent abnormalities of expression and function of E-cadherin and catenins, and associated disturbance of E-cadherin-mediated intercellular adhesion in gastric carcinoma cell lines. These findings support the tumour suppressor role of the E-cadherin and its contribution to the development and progression of the neoplastic phenotype in gastric carcinoma.

Original languageEnglish
Pages (from-to)322-330
Number of pages9
JournalBritish journal of cancer
Volume80
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1999

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Keywords

  • Cadherin
  • Catenin
  • Cell adhesion
  • Gastric carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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