Abnormalities of the cadherin-catenin complex in chemically-induced colo-rectal carcinogenesis

Emma Tucker, Andrea Buda, Nari Janghra, Jenny Baker, Joy Coad, Morganden Moorghan, Mike Havler, Peter Dettmar, Massimo Pignatelli

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

β-Catenin is a multifunctional protein originally identified as a component of the cadherin cellcell adhesion complex. It also binds the adenomatous polyposis coli (APC) tumour suppressor which controls β-catenin cellular levels through its degradation. β-Catenin and/or APC mutations result in increased cytoplasmic β-catenin and nuclear translocation. The aim of the present study was to examine the expression and cellular localisation of α- and β-catenin, p 120 and E-cadherin in a chemically-induced mouse model of colo-rectal cancer using 1,2-dimethylhydrazine (DMH). Female Balb/C mice were injected subcutaneously with a solution providing 25 mg DMH base/kg body weight for 17 weeks. Animals were killed and tumours identified in the intestine with a dissecting microscope. Formalin-fixed paraffin-embedded sections of normal and dysplastic colonic mucosa were stained by an indirect avidin-biotin immunohistochemical technique using mouse monoclonal antibodies, and membranous, cytoplasmic and nuclear cellular localisation was assessed by light microscopy. Staining distribution scored as follows: 3, >90 % positive epithelial cells; 2, >50 % positive epithelial cells; 1,

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalProceedings of the Nutrition Society
Volume62
Issue number1
DOIs
Publication statusPublished - Feb 2003
Externally publishedYes

Fingerprint

Catenins
cadherins
Cadherins
carcinogenesis
Carcinogenesis
epithelial cells
1,2-dimethylhydrazine
avidin
neoplasms
mice
biotin
colorectal neoplasms
formalin
Adenomatous Polyposis Coli
microscopes
alkanes
mucosa
adhesion
light microscopy
monoclonal antibodies

Keywords

  • Catenins
  • Colo-rectal carcinogenesis
  • Dimethylhydrazine model
  • E-cadherin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Abnormalities of the cadherin-catenin complex in chemically-induced colo-rectal carcinogenesis. / Tucker, Emma; Buda, Andrea; Janghra, Nari; Baker, Jenny; Coad, Joy; Moorghan, Morganden; Havler, Mike; Dettmar, Peter; Pignatelli, Massimo.

In: Proceedings of the Nutrition Society, Vol. 62, No. 1, 02.2003, p. 229-236.

Research output: Contribution to journalArticle

Tucker, E, Buda, A, Janghra, N, Baker, J, Coad, J, Moorghan, M, Havler, M, Dettmar, P & Pignatelli, M 2003, 'Abnormalities of the cadherin-catenin complex in chemically-induced colo-rectal carcinogenesis', Proceedings of the Nutrition Society, vol. 62, no. 1, pp. 229-236. https://doi.org/10.1079/PNS2003217
Tucker, Emma ; Buda, Andrea ; Janghra, Nari ; Baker, Jenny ; Coad, Joy ; Moorghan, Morganden ; Havler, Mike ; Dettmar, Peter ; Pignatelli, Massimo. / Abnormalities of the cadherin-catenin complex in chemically-induced colo-rectal carcinogenesis. In: Proceedings of the Nutrition Society. 2003 ; Vol. 62, No. 1. pp. 229-236.
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