TY - JOUR
T1 - Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB
AU - Aksenova, Vasilisa
AU - Turoverova, Lidia
AU - Khotin, Mikhai
AU - Magnusson, Karl Eric
AU - Tulchinsky, Eugene
AU - Melino, Gerry
AU - Pinaev, George P.
AU - Barlev, Nickolai
AU - Tentler, Dmitri
PY - 2013
Y1 - 2013
N2 - ACTN4 is an actin-binding protein that participates in cytoskeleton organisation. It resides both in the cytoplasm and nucleus and physically associates with various transcription factors. Here, we describe an effect of ACTN4 expression on transcriptional activity of the RelA/p65 subunit of NF-kB. We demonstrate that ACTN4 enhances RelA/p65-dependant expression of c-fos, MMP-3 and MMP-1 genes, but it does not affect TNC, ICAM1 and FN1 expression. Importantly, actin-binding domains of ACTN4 are not critical for the nuclear translocation and co-activation of RelA/p65-dependent transcription. Collectively, our data suggest that in the nucleus, ACTN4 functions as a selective transcriptional co-activator of RelA/p65.
AB - ACTN4 is an actin-binding protein that participates in cytoskeleton organisation. It resides both in the cytoplasm and nucleus and physically associates with various transcription factors. Here, we describe an effect of ACTN4 expression on transcriptional activity of the RelA/p65 subunit of NF-kB. We demonstrate that ACTN4 enhances RelA/p65-dependant expression of c-fos, MMP-3 and MMP-1 genes, but it does not affect TNC, ICAM1 and FN1 expression. Importantly, actin-binding domains of ACTN4 are not critical for the nuclear translocation and co-activation of RelA/p65-dependent transcription. Collectively, our data suggest that in the nucleus, ACTN4 functions as a selective transcriptional co-activator of RelA/p65.
KW - ACTN4
KW - MMP
KW - RelA/p65
KW - Transcription regulation
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U2 - 10.18632/oncotarget.901
DO - 10.18632/oncotarget.901
M3 - Article
AN - SCOPUS:84875791595
SN - 1949-2553
VL - 4
SP - 362
EP - 372
JO - Oncotarget
JF - Oncotarget
IS - 2
ER -