TY - JOUR
T1 - Activation of the NPTA element of the CYP2A3 gene by NFI-A2, a nasal mucosa-selective nuclear factor 1 isoform
AU - Xie, Yingqiu
AU - Madelian, Vergine
AU - Zhang, Jianhua
AU - Ling, Guoyu
AU - Ding, Xinxin
N1 - Funding Information:
We thank Dr. Alan Dahl for providing the cDNA library. The authors gratefully acknowledge the use of the Molecular Genetics and Yeast One/Two Hybrid Cores of the Wadsworth Center. This investigation was supported in part by NIH grant ES07462.
PY - 2001/12/21
Y1 - 2001/12/21
N2 - The aim of this study was to determine whether the NPTA element of the olfactory mucosa-predominant CYP2A3 gene can be activated by NFI-A2, a recently identified member of the nuclear factor 1 family of transcription factors. Isoform-specific RNA-PCR confirmed that NFI-A2 is mainly expressed in rat olfactory mucosa. A full-length NFI-A2 cDNA was isolated from a cDNA library of rat olfactory mucosa and was used for preparation of a construct encoding a fusion protein of NFI-A2 with the yeast GAL4 activation domain. Expression of the fusion protein in yeast was detected with an antibody to NFI-A. The fusion protein activated the expression of a LacZ reporter gene in yeast one-hybrid assays with a reporter construct containing the NPTA element, but not with other constructs lacking the NPTA element. These findings suggest that NFI-A2 may be involved in the tissue-selective transcriptional activation of the CYP2A3 gene in the olfactory mucosa.
AB - The aim of this study was to determine whether the NPTA element of the olfactory mucosa-predominant CYP2A3 gene can be activated by NFI-A2, a recently identified member of the nuclear factor 1 family of transcription factors. Isoform-specific RNA-PCR confirmed that NFI-A2 is mainly expressed in rat olfactory mucosa. A full-length NFI-A2 cDNA was isolated from a cDNA library of rat olfactory mucosa and was used for preparation of a construct encoding a fusion protein of NFI-A2 with the yeast GAL4 activation domain. Expression of the fusion protein in yeast was detected with an antibody to NFI-A. The fusion protein activated the expression of a LacZ reporter gene in yeast one-hybrid assays with a reporter construct containing the NPTA element, but not with other constructs lacking the NPTA element. These findings suggest that NFI-A2 may be involved in the tissue-selective transcriptional activation of the CYP2A3 gene in the olfactory mucosa.
UR - http://www.scopus.com/inward/record.url?scp=0035931007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035931007&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2001.6084
DO - 10.1006/bbrc.2001.6084
M3 - Article
C2 - 11741324
AN - SCOPUS:0035931007
VL - 289
SP - 1225
EP - 1228
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 5
ER -