Adenosine 5′ phosphosulfate reductase and sulfite oxidase regulate sulfite-induced water loss in Arabidopsis

Aizat Bekturova, DInara Oshanova, Poonam Tiwari, Zhadyrassyn Nurbekova, Assylay Kurmanbayeva, Aigerim Soltabayeva, Dmitry Yarmolinsky, Sudhakar Srivastava, Veronika Turecková, Miroslav Strnad, Moshe Sagi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Chloroplast-localized adenosine-5'-phosphosulphate reductase (APR) generates sulfite and plays a pivotal role in reduction of sulfate to cysteine. The peroxisome-localized sulfite oxidase (SO) oxidizes excess sulfite to sulfate. Arabidopsis wild type, SO RNA-interference (SO Ri) and SO overexpression (SO OE) transgenic lines infiltrated with sulfite showed increased water loss in SO Ri plants, and smaller stomatal apertures in SO OE plants compared with wild-type plants. Sulfite application also limited sulfate and abscisic acid-induced stomatal closure in wild type and SO Ri. The increases in APR activity in response to sulfite infiltration into wild type and SO Ri leaves resulted in an increase in endogenous sulfite, indicating that APR has an important role in sulfite-induced increases in stomatal aperture. Sulfite-induced H2O2 generation by NADPH oxidase led to enhanced APR expression and sulfite production. Suppression of APR by inhibiting NADPH oxidase and glutathione reductase2 (GR2), or mutation in APR2 or GR2, resulted in a decrease in sulfite production and stomatal apertures. The importance of APR and SO and the significance of sulfite concentrations in water loss were further demonstrated during rapid, harsh drought stress in root-detached wild-type, gr2 and SO transgenic plants. Our results demonstrate the role of SO in sulfite homeostasis in relation to water consumption in well-watered plants.

Original languageEnglish
Pages (from-to)6447-6466
Number of pages20
JournalJournal of Experimental Botany
Volume72
Issue number18
DOIs
Publication statusPublished - Sep 30 2021
Externally publishedYes

Keywords

  • Adenosine 5'-phosphosulfate reductase
  • DPI
  • glutathione reductase
  • reactive sulfur species
  • stomata
  • sulfite
  • sulfite oxidase

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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