Adsorption of proteins in channels of carbon nanotubes

Effect of surface chemistry

Marianna V. Kharlamova, Vadym N. Mochalin, Maria R. Lukatskaya, Junjie Niu, Volker Presser, Sergey Mikhalovsky, Yury Gogotsi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The adsorption of molecules in a confined environment (pores and narrow channels) differs from adsorption on flat surfaces. While the immobilization of proteins on porous carbon and the transport of protein molecules through carbon nanotube channels are of great practical importance, the interaction of proteins with the carbon surface in confinement is poorly understood. In this study the adsorption of bovine serum albumin (BSA) and tumor necrosis factor-α(TNF-α)was studied in carbon nanotubes grown by chemical vapor deposition in cylindrical pores of anodic alumina membranes. BSA adsorption depends on the channel diameter of the carbon nanotubes, the termination of nanotube surfaces (surface chemistry), and the pH of medium. Amination of the carbon surface leads to increased adsorption of the proteins at neutral pH, while oxidation decreases the sorption capacity. The differences have been explained by favorable or unfavorable electrostatic interactions between protein molecules and the carbon surface.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalMaterials Express
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Carbon Nanotubes
Surface chemistry
Carbon nanotubes
Proteins
Adsorption
Carbon
Bovine Serum Albumin
Molecules
Amination
Aluminum Oxide
Coulomb interactions
Nanotubes
Sorption
Chemical vapor deposition
Carrier Proteins
Alumina
Tumor Necrosis Factor-alpha
Membranes
Oxidation

Keywords

  • Anodic alumina membrane
  • Bovine serum albumin
  • Carbon nanotube
  • Protein adsorption
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Kharlamova, M. V., Mochalin, V. N., Lukatskaya, M. R., Niu, J., Presser, V., Mikhalovsky, S., & Gogotsi, Y. (2013). Adsorption of proteins in channels of carbon nanotubes: Effect of surface chemistry. Materials Express, 3(1), 1-10. https://doi.org/10.1166/mex.2013.1102

Adsorption of proteins in channels of carbon nanotubes : Effect of surface chemistry. / Kharlamova, Marianna V.; Mochalin, Vadym N.; Lukatskaya, Maria R.; Niu, Junjie; Presser, Volker; Mikhalovsky, Sergey; Gogotsi, Yury.

In: Materials Express, Vol. 3, No. 1, 03.2013, p. 1-10.

Research output: Contribution to journalArticle

Kharlamova, MV, Mochalin, VN, Lukatskaya, MR, Niu, J, Presser, V, Mikhalovsky, S & Gogotsi, Y 2013, 'Adsorption of proteins in channels of carbon nanotubes: Effect of surface chemistry', Materials Express, vol. 3, no. 1, pp. 1-10. https://doi.org/10.1166/mex.2013.1102
Kharlamova MV, Mochalin VN, Lukatskaya MR, Niu J, Presser V, Mikhalovsky S et al. Adsorption of proteins in channels of carbon nanotubes: Effect of surface chemistry. Materials Express. 2013 Mar;3(1):1-10. https://doi.org/10.1166/mex.2013.1102
Kharlamova, Marianna V. ; Mochalin, Vadym N. ; Lukatskaya, Maria R. ; Niu, Junjie ; Presser, Volker ; Mikhalovsky, Sergey ; Gogotsi, Yury. / Adsorption of proteins in channels of carbon nanotubes : Effect of surface chemistry. In: Materials Express. 2013 ; Vol. 3, No. 1. pp. 1-10.
@article{02ec3403143147f095c8be988f60ed09,
title = "Adsorption of proteins in channels of carbon nanotubes: Effect of surface chemistry",
abstract = "The adsorption of molecules in a confined environment (pores and narrow channels) differs from adsorption on flat surfaces. While the immobilization of proteins on porous carbon and the transport of protein molecules through carbon nanotube channels are of great practical importance, the interaction of proteins with the carbon surface in confinement is poorly understood. In this study the adsorption of bovine serum albumin (BSA) and tumor necrosis factor-α(TNF-α)was studied in carbon nanotubes grown by chemical vapor deposition in cylindrical pores of anodic alumina membranes. BSA adsorption depends on the channel diameter of the carbon nanotubes, the termination of nanotube surfaces (surface chemistry), and the pH of medium. Amination of the carbon surface leads to increased adsorption of the proteins at neutral pH, while oxidation decreases the sorption capacity. The differences have been explained by favorable or unfavorable electrostatic interactions between protein molecules and the carbon surface.",
keywords = "Anodic alumina membrane, Bovine serum albumin, Carbon nanotube, Protein adsorption, Tumor necrosis factor-α",
author = "Kharlamova, {Marianna V.} and Mochalin, {Vadym N.} and Lukatskaya, {Maria R.} and Junjie Niu and Volker Presser and Sergey Mikhalovsky and Yury Gogotsi",
year = "2013",
month = "3",
doi = "10.1166/mex.2013.1102",
language = "English",
volume = "3",
pages = "1--10",
journal = "Materials Express",
issn = "2158-5849",
publisher = "American Scientific Publishers",
number = "1",

}

TY - JOUR

T1 - Adsorption of proteins in channels of carbon nanotubes

T2 - Effect of surface chemistry

AU - Kharlamova, Marianna V.

AU - Mochalin, Vadym N.

AU - Lukatskaya, Maria R.

AU - Niu, Junjie

AU - Presser, Volker

AU - Mikhalovsky, Sergey

AU - Gogotsi, Yury

PY - 2013/3

Y1 - 2013/3

N2 - The adsorption of molecules in a confined environment (pores and narrow channels) differs from adsorption on flat surfaces. While the immobilization of proteins on porous carbon and the transport of protein molecules through carbon nanotube channels are of great practical importance, the interaction of proteins with the carbon surface in confinement is poorly understood. In this study the adsorption of bovine serum albumin (BSA) and tumor necrosis factor-α(TNF-α)was studied in carbon nanotubes grown by chemical vapor deposition in cylindrical pores of anodic alumina membranes. BSA adsorption depends on the channel diameter of the carbon nanotubes, the termination of nanotube surfaces (surface chemistry), and the pH of medium. Amination of the carbon surface leads to increased adsorption of the proteins at neutral pH, while oxidation decreases the sorption capacity. The differences have been explained by favorable or unfavorable electrostatic interactions between protein molecules and the carbon surface.

AB - The adsorption of molecules in a confined environment (pores and narrow channels) differs from adsorption on flat surfaces. While the immobilization of proteins on porous carbon and the transport of protein molecules through carbon nanotube channels are of great practical importance, the interaction of proteins with the carbon surface in confinement is poorly understood. In this study the adsorption of bovine serum albumin (BSA) and tumor necrosis factor-α(TNF-α)was studied in carbon nanotubes grown by chemical vapor deposition in cylindrical pores of anodic alumina membranes. BSA adsorption depends on the channel diameter of the carbon nanotubes, the termination of nanotube surfaces (surface chemistry), and the pH of medium. Amination of the carbon surface leads to increased adsorption of the proteins at neutral pH, while oxidation decreases the sorption capacity. The differences have been explained by favorable or unfavorable electrostatic interactions between protein molecules and the carbon surface.

KW - Anodic alumina membrane

KW - Bovine serum albumin

KW - Carbon nanotube

KW - Protein adsorption

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=84875730606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875730606&partnerID=8YFLogxK

U2 - 10.1166/mex.2013.1102

DO - 10.1166/mex.2013.1102

M3 - Article

VL - 3

SP - 1

EP - 10

JO - Materials Express

JF - Materials Express

SN - 2158-5849

IS - 1

ER -