Age-related changes in metallothionein, copper, copper-associated protein, and lipofuscin in human liver

A histochemical and immunohistochemical study

C. E. Fuller, M. E. Elmes, B. Jasani

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The relationship between immunohistochemically demonstrable hepatic metallothionein (MT) and age was studied in histologically normal livers from 12 fetuses (13-28 weeks' gestation) and 19 children (birth-4 years). In addition, the relationships between copper (rubeanic acid-positive), copper-associated protein (orcein-positive), lipofuscin (periodic acid Schiff after diastase digestion and Schmorls-positive), and MT (immunostaining) were studied. The fetal livers showed periportal and patchy mid-zone copper-associated protein and lipofuscin granules, together with strong diffuse cytoplasmic and nuclear MT immunostaining. On the other hand, the livers of the children over 6 months of age showed no copper-associated protein or lipofuscin granules, and minimal or no cytoplasmic MT immunostaining (one case showed nuclear MT immunostaining). The DPAS and Schmorls-positive lipofuscin granules appeared to be identical to the orcein-positive copper-associated protein granules which were consistently negative on MT immunostaining. The demonstration of cytoplasmic granular orcein positivity or diffuse non-granular MT immunostaining in liver above the age of 6 months would appear to be of pathological significance.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalJournal of Pathology
Volume161
Issue number2
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Lipofuscin
Metallothionein
Copper
Liver
Proteins
Periodic Acid
Amylases
copper thionein
Digestion
Fetus
Parturition
Pregnancy

Keywords

  • Copper
  • Copper-associated protein
  • Lipofuscin
  • Liver
  • Metallothionein
  • Neonate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Age-related changes in metallothionein, copper, copper-associated protein, and lipofuscin in human liver : A histochemical and immunohistochemical study. / Fuller, C. E.; Elmes, M. E.; Jasani, B.

In: Journal of Pathology, Vol. 161, No. 2, 1990, p. 167-172.

Research output: Contribution to journalArticle

@article{182f33eebf914f608e55146b6ad1407f,
title = "Age-related changes in metallothionein, copper, copper-associated protein, and lipofuscin in human liver: A histochemical and immunohistochemical study",
abstract = "The relationship between immunohistochemically demonstrable hepatic metallothionein (MT) and age was studied in histologically normal livers from 12 fetuses (13-28 weeks' gestation) and 19 children (birth-4 years). In addition, the relationships between copper (rubeanic acid-positive), copper-associated protein (orcein-positive), lipofuscin (periodic acid Schiff after diastase digestion and Schmorls-positive), and MT (immunostaining) were studied. The fetal livers showed periportal and patchy mid-zone copper-associated protein and lipofuscin granules, together with strong diffuse cytoplasmic and nuclear MT immunostaining. On the other hand, the livers of the children over 6 months of age showed no copper-associated protein or lipofuscin granules, and minimal or no cytoplasmic MT immunostaining (one case showed nuclear MT immunostaining). The DPAS and Schmorls-positive lipofuscin granules appeared to be identical to the orcein-positive copper-associated protein granules which were consistently negative on MT immunostaining. The demonstration of cytoplasmic granular orcein positivity or diffuse non-granular MT immunostaining in liver above the age of 6 months would appear to be of pathological significance.",
keywords = "Copper, Copper-associated protein, Lipofuscin, Liver, Metallothionein, Neonate",
author = "Fuller, {C. E.} and Elmes, {M. E.} and B. Jasani",
year = "1990",
doi = "10.1002/path.1711610212",
language = "English",
volume = "161",
pages = "167--172",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Age-related changes in metallothionein, copper, copper-associated protein, and lipofuscin in human liver

T2 - A histochemical and immunohistochemical study

AU - Fuller, C. E.

AU - Elmes, M. E.

AU - Jasani, B.

PY - 1990

Y1 - 1990

N2 - The relationship between immunohistochemically demonstrable hepatic metallothionein (MT) and age was studied in histologically normal livers from 12 fetuses (13-28 weeks' gestation) and 19 children (birth-4 years). In addition, the relationships between copper (rubeanic acid-positive), copper-associated protein (orcein-positive), lipofuscin (periodic acid Schiff after diastase digestion and Schmorls-positive), and MT (immunostaining) were studied. The fetal livers showed periportal and patchy mid-zone copper-associated protein and lipofuscin granules, together with strong diffuse cytoplasmic and nuclear MT immunostaining. On the other hand, the livers of the children over 6 months of age showed no copper-associated protein or lipofuscin granules, and minimal or no cytoplasmic MT immunostaining (one case showed nuclear MT immunostaining). The DPAS and Schmorls-positive lipofuscin granules appeared to be identical to the orcein-positive copper-associated protein granules which were consistently negative on MT immunostaining. The demonstration of cytoplasmic granular orcein positivity or diffuse non-granular MT immunostaining in liver above the age of 6 months would appear to be of pathological significance.

AB - The relationship between immunohistochemically demonstrable hepatic metallothionein (MT) and age was studied in histologically normal livers from 12 fetuses (13-28 weeks' gestation) and 19 children (birth-4 years). In addition, the relationships between copper (rubeanic acid-positive), copper-associated protein (orcein-positive), lipofuscin (periodic acid Schiff after diastase digestion and Schmorls-positive), and MT (immunostaining) were studied. The fetal livers showed periportal and patchy mid-zone copper-associated protein and lipofuscin granules, together with strong diffuse cytoplasmic and nuclear MT immunostaining. On the other hand, the livers of the children over 6 months of age showed no copper-associated protein or lipofuscin granules, and minimal or no cytoplasmic MT immunostaining (one case showed nuclear MT immunostaining). The DPAS and Schmorls-positive lipofuscin granules appeared to be identical to the orcein-positive copper-associated protein granules which were consistently negative on MT immunostaining. The demonstration of cytoplasmic granular orcein positivity or diffuse non-granular MT immunostaining in liver above the age of 6 months would appear to be of pathological significance.

KW - Copper

KW - Copper-associated protein

KW - Lipofuscin

KW - Liver

KW - Metallothionein

KW - Neonate

UR - http://www.scopus.com/inward/record.url?scp=0025312740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025312740&partnerID=8YFLogxK

U2 - 10.1002/path.1711610212

DO - 10.1002/path.1711610212

M3 - Article

VL - 161

SP - 167

EP - 172

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 2

ER -