AlGaN/GaN heterostructures for non-invasive cell electrophysiological measurements

Jinjiang Yu, Shrawan Kumar Jha, Lidan Xiao, Qingjun Liu, Ping Wang, Charles Surya, Mo Yang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recently, the ability to create bio-semiconductor hybrid devices has gained much interest for cell activity analysis. AlGaN material system has been demonstrated to be a promising cell-based biosensing platform due to a combination of unique properties, such as chemical inertness, optical transparency and low signal to noise ratios. To investigate the potential application of hybrid cell-AlGaN/GaN field effect transistor for cell electrophysiological monitoring, saos-2 human osteoblast-like cells were cultured in high density in non-metallized gate area of a transparent AlGaN/GaN heterostructure field effect transistor. We implemented and characterized the transistor recording of extracellular voltage in the cell-chip junction using the FET chip. The effect of ion channel blocker TEA on transistor signal was explored in order to test the capability of this hybrid chip for in vitro drug screening bioassay. Finally, the effect of cell adhesion on transistor signal was also studied by applying the protein kinase inhibitor H-7.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalBiosensors and Bioelectronics
Volume23
Issue number4
DOIs
Publication statusPublished - Nov 30 2007
Externally publishedYes

Fingerprint

Heterojunctions
Transistors
Field effect transistors
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Semiconductors
Preclinical Drug Evaluations
Intercellular Junctions
Hybrid Cells
Bioassay
Osteoblasts
Cell adhesion
Signal-To-Noise Ratio
High electron mobility transistors
Protein Kinase Inhibitors
Ion Channels
Cell Adhesion
Biological Assay
Transparency
Cultured Cells
Signal to noise ratio

Keywords

  • Extracellular recording
  • Field effect transistor
  • Ion channels

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

AlGaN/GaN heterostructures for non-invasive cell electrophysiological measurements. / Yu, Jinjiang; Jha, Shrawan Kumar; Xiao, Lidan; Liu, Qingjun; Wang, Ping; Surya, Charles; Yang, Mo.

In: Biosensors and Bioelectronics, Vol. 23, No. 4, 30.11.2007, p. 513-519.

Research output: Contribution to journalArticle

Yu, Jinjiang ; Jha, Shrawan Kumar ; Xiao, Lidan ; Liu, Qingjun ; Wang, Ping ; Surya, Charles ; Yang, Mo. / AlGaN/GaN heterostructures for non-invasive cell electrophysiological measurements. In: Biosensors and Bioelectronics. 2007 ; Vol. 23, No. 4. pp. 513-519.
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