Allelic diversity of acetyl coenzyme a carboxylase accd/bccp genes implicated in nuclear-cytoplasmic conflict in the wild and domesticated pea (Pisum sp.)

Eliška Nováková, Lenka Zablatzká, Jan Brus, Viktorie Nesrstová, Pavel Hanáček, Ruslan Kalendar, Fatima Cvrčková, L’Uboš Majeský, Petr Smýkal

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.

Original languageEnglish
Article number1773
JournalInternational journal of molecular sciences
Volume20
Issue number7
DOIs
Publication statusPublished - Apr 1 2019
Externally publishedYes

Keywords

  • Acetyl-CoA carboxylase
  • Hybrid incompatibility
  • Hybrid necrosis
  • Nuclear-cytoplasmic conflict
  • Pea
  • Reproductive isolation
  • Speciation

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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