Allopurinol provides long-term protection for experimentally induced testicular torsion in a rabbit model

Elijah O. Kehinde, Jehoram T. Anim, Olusegun A. Mojiminiyi, Farida Al-Awadi, Aida Shihab-Eldeen, Alexander E. Omu, Tunde Fatinikun, Asha Prasad, Mathew Abraham

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

OBJECTIVE: To assess the effect of five antioxidants on exocrine function of rabbit testes retained in situ for 24 h and 3 months after experimental torsion. MATERIALS AND METHODS: The left testes of peripubertal rabbits were clamped for 60 min, after which the clamps were removed and the testes allowed to reperfuse. The right testes served as interna controls. There were eight rabbits in each of the following experimental groups: (a) sham; (b) 60-min ischaemia followed by reperfusion; (c) 60-min ischaemia followed by left orchidectomy. In five further groups, rabbits were exposed to 60-min ischaemia followed by reperfusion, but received one of the following antioxidants before reperfusion: acetyl salicylic acid, ascorbic acid, allopurinol, quercetin or superoxide dismutase. Both testes were excised at 24 h or 3 months. The degree of lipid peroxidation.a measure of free radical damage, was assessed in testicular tissue homogenates by measuring the tissue evels of malondialdehyde (MDA). The Johnsen score was used to assess the morphologica damage at 24 h and 3 months for each group. RESULTS: At 3 months twisted viable testes allowed to reperfuse had higher MDA levels than controls; the left testes of rabbits treated with allopurinol had significantly lower MDA levels than untreated rabbitsand rabbitsgiven other antioxidants. Rabbits given quercetin, ascorbic acid or superoxide dismutase had lower (but not significantly) left testicular MDA levels than untreated rabbits, while rabbits given acetyl salicylic acid had even higher levels. Allopurinol-treated rabbits had a Johnsen score of > 7.6 and those given other antioxidants had scores of <7.6 at 3 months. CONCLUSION: The twisted viable testis treated by orchidopexy contains high free radical levels at 3 months. Of the antioxidants studied, only allopurinol had a beneficial long-term effect, by significantly reducing testicular MDA levels at 3 months.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBJU International
Volume96
Issue number1
DOIs
Publication statusPublished - Jul 2005
Externally publishedYes

Fingerprint

Spermatic Cord Torsion
Allopurinol
Testis
Rabbits
Malondialdehyde
Antioxidants
Reperfusion
Salicylic Acid
Ischemia
Quercetin
Ascorbic Acid
Superoxide Dismutase
Free Radicals
Orchiopexy
Orchiectomy
Lipid Peroxidation

Keywords

  • Antioxidants
  • Long-term results
  • Testicular torsion
  • Treatment

ASJC Scopus subject areas

  • Urology

Cite this

Kehinde, E. O., Anim, J. T., Mojiminiyi, O. A., Al-Awadi, F., Shihab-Eldeen, A., Omu, A. E., ... Abraham, M. (2005). Allopurinol provides long-term protection for experimentally induced testicular torsion in a rabbit model. BJU International, 96(1), 175-180. https://doi.org/10.1111/j.1464-410X.2005.05590.x

Allopurinol provides long-term protection for experimentally induced testicular torsion in a rabbit model. / Kehinde, Elijah O.; Anim, Jehoram T.; Mojiminiyi, Olusegun A.; Al-Awadi, Farida; Shihab-Eldeen, Aida; Omu, Alexander E.; Fatinikun, Tunde; Prasad, Asha; Abraham, Mathew.

In: BJU International, Vol. 96, No. 1, 07.2005, p. 175-180.

Research output: Contribution to journalArticle

Kehinde, EO, Anim, JT, Mojiminiyi, OA, Al-Awadi, F, Shihab-Eldeen, A, Omu, AE, Fatinikun, T, Prasad, A & Abraham, M 2005, 'Allopurinol provides long-term protection for experimentally induced testicular torsion in a rabbit model', BJU International, vol. 96, no. 1, pp. 175-180. https://doi.org/10.1111/j.1464-410X.2005.05590.x
Kehinde, Elijah O. ; Anim, Jehoram T. ; Mojiminiyi, Olusegun A. ; Al-Awadi, Farida ; Shihab-Eldeen, Aida ; Omu, Alexander E. ; Fatinikun, Tunde ; Prasad, Asha ; Abraham, Mathew. / Allopurinol provides long-term protection for experimentally induced testicular torsion in a rabbit model. In: BJU International. 2005 ; Vol. 96, No. 1. pp. 175-180.
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abstract = "OBJECTIVE: To assess the effect of five antioxidants on exocrine function of rabbit testes retained in situ for 24 h and 3 months after experimental torsion. MATERIALS AND METHODS: The left testes of peripubertal rabbits were clamped for 60 min, after which the clamps were removed and the testes allowed to reperfuse. The right testes served as interna controls. There were eight rabbits in each of the following experimental groups: (a) sham; (b) 60-min ischaemia followed by reperfusion; (c) 60-min ischaemia followed by left orchidectomy. In five further groups, rabbits were exposed to 60-min ischaemia followed by reperfusion, but received one of the following antioxidants before reperfusion: acetyl salicylic acid, ascorbic acid, allopurinol, quercetin or superoxide dismutase. Both testes were excised at 24 h or 3 months. The degree of lipid peroxidation.a measure of free radical damage, was assessed in testicular tissue homogenates by measuring the tissue evels of malondialdehyde (MDA). The Johnsen score was used to assess the morphologica damage at 24 h and 3 months for each group. RESULTS: At 3 months twisted viable testes allowed to reperfuse had higher MDA levels than controls; the left testes of rabbits treated with allopurinol had significantly lower MDA levels than untreated rabbitsand rabbitsgiven other antioxidants. Rabbits given quercetin, ascorbic acid or superoxide dismutase had lower (but not significantly) left testicular MDA levels than untreated rabbits, while rabbits given acetyl salicylic acid had even higher levels. Allopurinol-treated rabbits had a Johnsen score of > 7.6 and those given other antioxidants had scores of <7.6 at 3 months. CONCLUSION: The twisted viable testis treated by orchidopexy contains high free radical levels at 3 months. Of the antioxidants studied, only allopurinol had a beneficial long-term effect, by significantly reducing testicular MDA levels at 3 months.",
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AU - Mojiminiyi, Olusegun A.

AU - Al-Awadi, Farida

AU - Shihab-Eldeen, Aida

AU - Omu, Alexander E.

AU - Fatinikun, Tunde

AU - Prasad, Asha

AU - Abraham, Mathew

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N2 - OBJECTIVE: To assess the effect of five antioxidants on exocrine function of rabbit testes retained in situ for 24 h and 3 months after experimental torsion. MATERIALS AND METHODS: The left testes of peripubertal rabbits were clamped for 60 min, after which the clamps were removed and the testes allowed to reperfuse. The right testes served as interna controls. There were eight rabbits in each of the following experimental groups: (a) sham; (b) 60-min ischaemia followed by reperfusion; (c) 60-min ischaemia followed by left orchidectomy. In five further groups, rabbits were exposed to 60-min ischaemia followed by reperfusion, but received one of the following antioxidants before reperfusion: acetyl salicylic acid, ascorbic acid, allopurinol, quercetin or superoxide dismutase. Both testes were excised at 24 h or 3 months. The degree of lipid peroxidation.a measure of free radical damage, was assessed in testicular tissue homogenates by measuring the tissue evels of malondialdehyde (MDA). The Johnsen score was used to assess the morphologica damage at 24 h and 3 months for each group. RESULTS: At 3 months twisted viable testes allowed to reperfuse had higher MDA levels than controls; the left testes of rabbits treated with allopurinol had significantly lower MDA levels than untreated rabbitsand rabbitsgiven other antioxidants. Rabbits given quercetin, ascorbic acid or superoxide dismutase had lower (but not significantly) left testicular MDA levels than untreated rabbits, while rabbits given acetyl salicylic acid had even higher levels. Allopurinol-treated rabbits had a Johnsen score of > 7.6 and those given other antioxidants had scores of <7.6 at 3 months. CONCLUSION: The twisted viable testis treated by orchidopexy contains high free radical levels at 3 months. Of the antioxidants studied, only allopurinol had a beneficial long-term effect, by significantly reducing testicular MDA levels at 3 months.

AB - OBJECTIVE: To assess the effect of five antioxidants on exocrine function of rabbit testes retained in situ for 24 h and 3 months after experimental torsion. MATERIALS AND METHODS: The left testes of peripubertal rabbits were clamped for 60 min, after which the clamps were removed and the testes allowed to reperfuse. The right testes served as interna controls. There were eight rabbits in each of the following experimental groups: (a) sham; (b) 60-min ischaemia followed by reperfusion; (c) 60-min ischaemia followed by left orchidectomy. In five further groups, rabbits were exposed to 60-min ischaemia followed by reperfusion, but received one of the following antioxidants before reperfusion: acetyl salicylic acid, ascorbic acid, allopurinol, quercetin or superoxide dismutase. Both testes were excised at 24 h or 3 months. The degree of lipid peroxidation.a measure of free radical damage, was assessed in testicular tissue homogenates by measuring the tissue evels of malondialdehyde (MDA). The Johnsen score was used to assess the morphologica damage at 24 h and 3 months for each group. RESULTS: At 3 months twisted viable testes allowed to reperfuse had higher MDA levels than controls; the left testes of rabbits treated with allopurinol had significantly lower MDA levels than untreated rabbitsand rabbitsgiven other antioxidants. Rabbits given quercetin, ascorbic acid or superoxide dismutase had lower (but not significantly) left testicular MDA levels than untreated rabbits, while rabbits given acetyl salicylic acid had even higher levels. Allopurinol-treated rabbits had a Johnsen score of > 7.6 and those given other antioxidants had scores of <7.6 at 3 months. CONCLUSION: The twisted viable testis treated by orchidopexy contains high free radical levels at 3 months. Of the antioxidants studied, only allopurinol had a beneficial long-term effect, by significantly reducing testicular MDA levels at 3 months.

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