Altered expression and function of E-cadherin in cervical intraepithelial neoplasia and invasive squamous cell carcinoma

C. J. Vessey, J. Wilding, N. Folarin, S. Hirano, M. Takeichi, P. Soutter, G. W H Stamp, M. Pignatelli

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

HECD-1 monoclonal antibody has been used to localize E-cadherin, a calcium-dependent cell-cell adhesion molecule, in microwave-treated, paraffin-embedded sections from 53 cases of cervical intraepithelial neoplasia (CIN) (11 CIN I, 22 CIN II, and 20 CIN III), 16 invasive cervical squamous cell carcinomas, and seven metastases. In normal cervix, E-cadherin was expressed on the cell membrane of basal and parabasal cells. Cytoplasmic staining was present in occasional basal cells only. In CIN, the presence and localization of cytoplasmic E-cadherin were found to be significantly correlated with the grade of the CIN lesion. In squamous cell carcinomas, reduced membranous and increased cytoplasmic staining was seen with worsening differentiation. Loss of membranous E-cadherin expression was also detected in 4/7 metastatic deposits. E-cadherin expression (120 kD form, on Western blotting) was seen in human cervical carcinoma cell lines (HT3, ME180, C4I, Caski) that maintained the ability to aggregate in a homotypic adhesion assay and showed a typical epithelial morphology. E-cadherin-negative cell lines (Hela, SiHa, C33A) did not show adhesion. HOG-1 was the only E-cadherin-negative cell line which showed a significant degree of cell-tell aggregation. These data indicate that loss of membranous E-cadherin expression may represent one of the abnormalities underlying loss of cell polarity and differentiation which characterize CIN and invasive cervical cancer.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalJournal of Pathology
Volume176
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

    Fingerprint

Keywords

  • Adhesion
  • Cervix
  • E-cadherin
  • Invasion
  • Metastasis
  • Polarity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this