An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion

James J. Faust, Arnat Balabiyev, John M. Heddleston, Nataly P. Podolnikova, D. Page Baluch, Teng Leong Chew, Tatiana P. Ugarova

Research output: Contribution to journalArticle

Abstract

Macrophage fusion resulting in the formation of multinucleated giant cells occurs in a variety of chronic inflammatory diseases, yet the mechanism responsible for initiating this process is unknown. Here, we used live cell imaging to show that actin-based protrusions at the leading edge initiate macrophage fusion. Phase-contrast video microscopy demonstrated that in the majority of events, short protrusions (∼3 µm) between two closely apposed cells initiated fusion, but occasionally we observed long protrusions (∼12 µm). Using macrophages isolated from LifeAct mice and imaging with lattice light sheet microscopy, we further found that fusion-competent protrusions formed at sites enriched in podosomes. Inducing fusion in mixed populations of GFP- and mRFP-LifeAct macrophages showed rapid spatial overlap between GFP and RFP signal at the site of fusion. Cytochalasin B strongly reduced fusion and when rare fusion events occurred, protrusions were not observed. Fusion of macrophages deficient in Wiskott-Aldrich syndrome protein and Cdc42, key molecules involved in the formation of actin-based protrusions and podosomes, was also impaired both in vitro and in vivo. Finally, inhibiting the activity of the Arp2/3 complex decreased fusion and podosome formation. Together these data suggest that an actin-based protrusion formed at the leading edge initiates macrophage fusion.

Original languageEnglish
Pages (from-to)2254-2267
Number of pages14
JournalMolecular biology of the cell
Volume30
Issue number17
DOIs
Publication statusPublished - Aug 1 2019

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Actins
Macrophages
Wiskott-Aldrich Syndrome Protein
Actin-Related Protein 2-3 Complex
Phase-Contrast Microscopy
Video Microscopy
Cytochalasin B
Cell Fusion
Giant Cells
Podosomes
Microscopy
Chronic Disease
Light
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Faust, J. J., Balabiyev, A., Heddleston, J. M., Podolnikova, N. P., Baluch, D. P., Chew, T. L., & Ugarova, T. P. (2019). An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion. Molecular biology of the cell, 30(17), 2254-2267. https://doi.org/10.1091/mbc.E19-01-0009

An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion. / Faust, James J.; Balabiyev, Arnat; Heddleston, John M.; Podolnikova, Nataly P.; Baluch, D. Page; Chew, Teng Leong; Ugarova, Tatiana P.

In: Molecular biology of the cell, Vol. 30, No. 17, 01.08.2019, p. 2254-2267.

Research output: Contribution to journalArticle

Faust, JJ, Balabiyev, A, Heddleston, JM, Podolnikova, NP, Baluch, DP, Chew, TL & Ugarova, TP 2019, 'An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion', Molecular biology of the cell, vol. 30, no. 17, pp. 2254-2267. https://doi.org/10.1091/mbc.E19-01-0009
Faust JJ, Balabiyev A, Heddleston JM, Podolnikova NP, Baluch DP, Chew TL et al. An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion. Molecular biology of the cell. 2019 Aug 1;30(17):2254-2267. https://doi.org/10.1091/mbc.E19-01-0009
Faust, James J. ; Balabiyev, Arnat ; Heddleston, John M. ; Podolnikova, Nataly P. ; Baluch, D. Page ; Chew, Teng Leong ; Ugarova, Tatiana P. / An actin-based protrusion originating from a podosome-enriched region initiates macrophage fusion. In: Molecular biology of the cell. 2019 ; Vol. 30, No. 17. pp. 2254-2267.
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