An ancient player unmasked

T4 rl encodes a t-specific antiholin

E. Ramanculov, R. Young

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Phage T4 effects lysis by its holin T and its endolysin E. Lysis is inhibited (LIN) if the infected cell is subjected to secondary infections by T4 phage particles. The T4 rl gene is required for LIN in all hosts tested. Here, we show that a cloned rl gene can impose a T-specific LIN on T-mediated lysis in the context of the phage λ infective cycle, in the absence of other T4 genes and without secondary infection by T4. Moreover, it is shown that the T holin accumulates in the membrane during LIN, forming SDS-resistant oligomers. We show by cross-linking experiments that a T-RI heterodimer is formed during LIN, demonstrating that RI belongs to the functional class of antiholins, such as the S107 protein of λ, which heterodimerizes with its cognate holin, S105. Finally, we show that the addition of Ni2+ ions to the medium can block lysis by a T protein hexahistidine-tagged at its C-terminus, suggesting that liganding of the periplasmic domain is sufficient to impose lysis inhibition. The results are discussed in terms of a model in which the LIN-inducing signal of the secondary infecting phage influences a conformational equilibrium assumed by RI in the periplasm.

Original languageEnglish
Pages (from-to)575-583
Number of pages9
JournalMolecular Microbiology
Volume41
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Bacteriophage T4
His-His-His-His-His-His
Coinfection
Bacteriophages
Genes
Periplasm
Proteins
Ions
Membranes
endolysin

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

An ancient player unmasked : T4 rl encodes a t-specific antiholin. / Ramanculov, E.; Young, R.

In: Molecular Microbiology, Vol. 41, No. 3, 2001, p. 575-583.

Research output: Contribution to journalArticle

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