TY - JOUR
T1 - An investigation of liquid chromatography-mass spectral attributes and analytical performance characteristics of tenofovir, emtricitabine and efavirenz in human plasma
AU - Ndolo, Sedireng M.
AU - Sichilongo, Kwenga
AU - Massele, Amos
AU - Sepako, Enoch
AU - Vento, Sandro
N1 - Publisher Copyright:
© The Author 2015. Published by Oxford University Press. All rights reserved.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Liquid chromatography (LC) and mass spectral behavior and analytical performance characteristics of efavirenz (EFV), emtricitabine (EMT) and tenofovir (TFV), i.e., individual components of Atripla®, were probed. This was followed by estimation of their analytical performance characteristics employing LC and a parallel direct infusion sample introduction procedure. Performance characteristics using both types of sample introduction procedures were compared. Using liquid chromatography-mass spectrometry (LC-MS), linearities, i.e., correlation coefficients of the calibration curves of EFV, EMT and TFV, ranged between 0.9300 and 0.9990 in the full scan, selected ion monitoring and mass spectrometry/mass spectrometry (MS-MS) modes. The limits of detection (LODs) ranged between 0.5 and 11.6 μg/L. The lower limits of quantification (LLOQs) and the upper limits of quantification (ULOQs) were in the ranges of 0.9-23.2 and 1.6-38.7 μg/L, respectively. The LODs ranged between 0.8 and 114.7 μg/L. The LLOQs and the ULOQs were in the ranges of 1.6-29.4 and 2.7-49.0 μg/L, respectively. In the case of EMT, sodiated molecular ion at m/z 270 was used to adduce analytical performance characteristics from which lower detection limits were obtained compared with those in the literature where [M+H]+ at m/z 248 was used.
AB - Liquid chromatography (LC) and mass spectral behavior and analytical performance characteristics of efavirenz (EFV), emtricitabine (EMT) and tenofovir (TFV), i.e., individual components of Atripla®, were probed. This was followed by estimation of their analytical performance characteristics employing LC and a parallel direct infusion sample introduction procedure. Performance characteristics using both types of sample introduction procedures were compared. Using liquid chromatography-mass spectrometry (LC-MS), linearities, i.e., correlation coefficients of the calibration curves of EFV, EMT and TFV, ranged between 0.9300 and 0.9990 in the full scan, selected ion monitoring and mass spectrometry/mass spectrometry (MS-MS) modes. The limits of detection (LODs) ranged between 0.5 and 11.6 μg/L. The lower limits of quantification (LLOQs) and the upper limits of quantification (ULOQs) were in the ranges of 0.9-23.2 and 1.6-38.7 μg/L, respectively. The LODs ranged between 0.8 and 114.7 μg/L. The LLOQs and the ULOQs were in the ranges of 1.6-29.4 and 2.7-49.0 μg/L, respectively. In the case of EMT, sodiated molecular ion at m/z 270 was used to adduce analytical performance characteristics from which lower detection limits were obtained compared with those in the literature where [M+H]+ at m/z 248 was used.
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U2 - 10.1093/jat/bkv119
DO - 10.1093/jat/bkv119
M3 - Article
C2 - 26487641
AN - SCOPUS:84960106412
VL - 40
SP - 49
EP - 57
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
SN - 0146-4760
IS - 1
M1 - bkv119
ER -