Abstract
A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
| Original language | English |
|---|---|
| Pages (from-to) | 1271-1284 |
| Number of pages | 14 |
| Journal | Journal of Proteome Research |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 6 2009 |
| Externally published | Yes |
Keywords
- Biogenic amine
- Dopamine
- Drosophila
- MALDI-TOF
- Mass spectrometry
- Neuropeptide
- Serotonin
ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
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Cite this
Yew, J. Y., Wang, Y., Barteneva, N., Dikler, S., Kutz-Naber, K. K., Li, N., & Kravitz, E. A. (2009). Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry. Journal of Proteome Research, 8(3), 1271-1284. https://doi.org/10.1021/pr800601x