Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry

Joanne Y. Yew; Yun Wang; Natasha Barteneva; Sergei Dikler; Kimberly K. Kutz-Naber; Ngjun Li; Edward A. Kravitz

doi:10.1021/pr800601x

Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry

Joanne Y. Yew, Yun Wang, Natasha Barteneva, Sergei Dikler, Kimberly K. Kutz-Naber, Ngjun Li, Edward A. Kravitz

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.

Original language	English
Pages (from-to)	1271-1284
Number of pages	14
Journal	Journal of Proteome Research
Volume	8
Issue number	3
DOIs	https://doi.org/10.1021/pr800601x
Publication status	Published - Mar 6 2009
Externally published	Yes

Keywords

Biogenic amine
Dopamine
Drosophila
MALDI-TOF
Mass spectrometry
Neuropeptide
Serotonin

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

Access to Document

10.1021/pr800601x

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Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry. / Yew, Joanne Y.; Wang, Yun; Barteneva, Natasha; Dikler, Sergei; Kutz-Naber, Kimberly K.; Li, Ngjun; Kravitz, Edward A.

In: Journal of Proteome Research, Vol. 8, No. 3, 06.03.2009, p. 1271-1284.

Research output: Contribution to journal › Article › peer-review

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