Abstract
A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
| Original language | English |
|---|---|
| Pages (from-to) | 1271-1284 |
| Number of pages | 14 |
| Journal | Journal of Proteome Research |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 6 2009 |
| Externally published | Yes |
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Keywords
- Biogenic amine
- Dopamine
- Drosophila
- MALDI-TOF
- Mass spectrometry
- Neuropeptide
- Serotonin
ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
Cite this
Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry. / Yew, Joanne Y.; Wang, Yun; Barteneva, Natasha; Dikler, Sergei; Kutz-Naber, Kimberly K.; Li, Ngjun; Kravitz, Edward A.
In: Journal of Proteome Research, Vol. 8, No. 3, 06.03.2009, p. 1271-1284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry
AU - Yew, Joanne Y.
AU - Wang, Yun
AU - Barteneva, Natasha
AU - Dikler, Sergei
AU - Kutz-Naber, Kimberly K.
AU - Li, Ngjun
AU - Kravitz, Edward A.
PY - 2009/3/6
Y1 - 2009/3/6
N2 - A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
AB - A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
KW - Biogenic amine
KW - Dopamine
KW - Drosophila
KW - MALDI-TOF
KW - Mass spectrometry
KW - Neuropeptide
KW - Serotonin
UR - http://www.scopus.com/inward/record.url?scp=65249137648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65249137648&partnerID=8YFLogxK
U2 - 10.1021/pr800601x
DO - 10.1021/pr800601x
M3 - Article
C2 - 19199706
AN - SCOPUS:65249137648
VL - 8
SP - 1271
EP - 1284
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 3
ER -