Analysis of neuropeptide expression and localization in adult Drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry

Joanne Y. Yew, Yun Wang, Natasha Barteneva, Sergei Dikler, Kimberly K. Kutz-Naber, Ngjun Li, Edward A. Kravitz

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.

Original languageEnglish
Pages (from-to)1271-1284
Number of pages14
JournalJournal of Proteome Research
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 6 2009
Externally publishedYes

Keywords

  • Biogenic amine
  • Dopamine
  • Drosophila
  • MALDI-TOF
  • Mass spectrometry
  • Neuropeptide
  • Serotonin

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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