AIM: To study the anti-inflammatory effects and the action mechanisms of berberine. METHODS: Leukocytes chemotaxis; chemiluminscence with respiratory burst of polymorphonuclears in vitro; microacidimetry of PLA2 activity; ultraviolet spectrometry of PGE2 in inflammatory footpad of rats. RESULTS: The Ber with 5 X 10-5. 5 X 10-4 mol · L-1 remarkably reduced the leukocytes chemotaxis induced by ZAP (zymosan actived plasma) in vitro and reduced its random motility in large content, inhibited luminol-dependent chemiluminscence (CL) produced by respiratory burst of rat polymorphonclears (PMNs). Ber also inhibited the CL produced by VitC - Cu2+, zymosan and release of hydrogen perxides with 5 X 10-4 5 X 10-5 mol · L-1 respectively. The phospholipase A2 (PLA2) activity of PMNs was decreased when incubated with Ber 1 h. Ber reduced the content of PGE2, in inflammatory tissue induced by carrageenin in vivo. CONCLUSIONS: The anti- inflammatory mechanism of Ber may involve in several paths.
|Number of pages||4|
|Journal||Chinese Pharmacological Bulletin|
|Publication status||Published - Jan 1 1998|
- Phospholipase A
- Prostaglandin E
ASJC Scopus subject areas