ANTIGEN SPECIFIC SUPPRESSOR CELL FUNCTION IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS

Sandro Vento, Gianfranco Bottazzo, Roger Williams, JohnE Hegarty, Enrico Macchia, AdrianL W F Eddleston

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Abstract

An indirect migration inhibition assay has been used to show that lymphocytes from 26 of 29 patients with autoimmune chronic active hepatitis (CAH) generated T lymphocyte migration inhibitory factors (T-LIF) in the presence of liver specific protein (LSP), compared with only 1 of 21 patients with HBsAg-positive chronic liver disease and none of 19 controls. Generation of T-LIF activity in response to LSP was not observed in any of 5 patients with autoimmune thyroid disease although their T lymphocytes did generate T-LIF activity in the presence of thyroid membrane antigens. T lymphocytes from 1 patient with autoimmune liver and thyroid disease generated T-LIF activity in the presence of both LSP and thyroid membrane antigens. The generation of T-LIF activity by T cells from autoimmune CAH patients was suppressed when these cells were co-cultured in a 9:1 ratio with T cells from normal subjects and patients with HBsAg-positive chronic liver disease, but was unaffected if co-cultured with T cells from other patients with autoimmune CAH. T cells from patients with autoimmune CAH did, however, suppress the generation of T-LIF activity by T lymphocytes from patients with autoimmune thyroid disease when these cells were cultured with thyroid membrane antigens. After pretreatment with cimetidine or mitomycin-C for 30 min, T cells from normal subjects lost their ability to inhibit the generation of T-LIF activity to T lymphocytes from autoimmune CAH patients. These results are consistent with the hypothesis that there exists a defect in the specific suppressor T cell population controlling the immune response to LSP in autoimmune CAH which is unaffected by disease activity and treatment and which may be of fundamental importance in the pathogenesis of the disease.

Original languageEnglish
Pages (from-to)1200-1204
Number of pages5
JournalThe Lancet
Volume323
Issue number8388
DOIs
Publication statusPublished - Jun 2 1984
Externally publishedYes

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Autoimmune Hepatitis
Chronic Hepatitis
T-Lymphocytes
Antigens
Thyroid Diseases
Autoimmune Diseases
Liver Diseases
Thyroid Gland
Liver
Hepatitis B Surface Antigens
Chronic Disease
TCF Transcription Factors
Proteins
Membranes
Cimetidine
Mitomycin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Vento, S., Bottazzo, G., Williams, R., Hegarty, J., Macchia, E., & Eddleston, A. W. F. (1984). ANTIGEN SPECIFIC SUPPRESSOR CELL FUNCTION IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS. The Lancet, 323(8388), 1200-1204. https://doi.org/10.1016/S0140-6736(84)91691-X

ANTIGEN SPECIFIC SUPPRESSOR CELL FUNCTION IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS. / Vento, Sandro; Bottazzo, Gianfranco; Williams, Roger; Hegarty, JohnE; Macchia, Enrico; Eddleston, AdrianL W F.

In: The Lancet, Vol. 323, No. 8388, 02.06.1984, p. 1200-1204.

Research output: Contribution to journalArticle

Vento, S, Bottazzo, G, Williams, R, Hegarty, J, Macchia, E & Eddleston, AWF 1984, 'ANTIGEN SPECIFIC SUPPRESSOR CELL FUNCTION IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS', The Lancet, vol. 323, no. 8388, pp. 1200-1204. https://doi.org/10.1016/S0140-6736(84)91691-X
Vento S, Bottazzo G, Williams R, Hegarty J, Macchia E, Eddleston AWF. ANTIGEN SPECIFIC SUPPRESSOR CELL FUNCTION IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS. The Lancet. 1984 Jun 2;323(8388):1200-1204. https://doi.org/10.1016/S0140-6736(84)91691-X
Vento, Sandro ; Bottazzo, Gianfranco ; Williams, Roger ; Hegarty, JohnE ; Macchia, Enrico ; Eddleston, AdrianL W F. / ANTIGEN SPECIFIC SUPPRESSOR CELL FUNCTION IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS. In: The Lancet. 1984 ; Vol. 323, No. 8388. pp. 1200-1204.
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