Application of BARE-1 retrotransposon markers to the mapping of a major resistance gene for net blotch in barley

O. Manninen, R. Kalendar, J. Robinson, A. H. Schulman

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)

Abstract

Net blotch, which is caused by the fungus Pyrenophora teres Drechs. f. teres Smedeg., presents a serious problem for barley production worldwide, and the identification and deployment of sources of resistance to it are key objectives for many breeders. Here, we report the identification of a major resistance gene, accounting for 65% of the response variation, in a cross between the resistant line CI9819 and the susceptible cv. Rolfi. The resistance gene was mapped to chromosome 6H with the aid of two recently developed systems of retrotransposon-based molecular markers, REMAP and IRAP. A total of 239 BARE-1 and Sukkula retrotransposon markers were mapped in the cross, and the 30-cM segment containing the locus with significant resistance effect contained 26 of the markers. The type and local density of the markers should facilitate future map-based cloning of the resistance gene as well as manipulation of the resistance through backcross breeding.

Original languageEnglish
Pages (from-to)325-334
Number of pages10
JournalMolecular and General Genetics
Volume264
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • BARE-1 retrotransposon
  • Barley
  • Linkage mapping
  • Net blotch resistance
  • Quantitative trait locus (QTL)

ASJC Scopus subject areas

  • Genetics

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