Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women

Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova, Zaurech Abugalieva, Korlukain Seksembayeva, Syed Ali, Azliyati Azizan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives Human papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. Methods Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. Results HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. Conclusions Overall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalInternational Journal of Infectious Diseases
Volume54
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Kazakhstan
Papanicolaou Test
Cell Biology
Uterine Cervical Neoplasms
Human papillomavirus 31
Uterine Cervical Dysplasia
Human papillomavirus 16
Multiplex Polymerase Chain Reaction
Health Policy
Ambulatory Care Facilities
Real-Time Polymerase Chain Reaction
Genotype
DNA
Infection

Keywords

  • cervical cancer
  • CIN
  • HPV
  • Kazakhstan
  • prevalence

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women. / Niyazmetova, Luiza; Aimagambetova, Gulzhanat; Stambekova, Nazigul; Abugalieva, Zaurech; Seksembayeva, Korlukain; Ali, Syed; Azizan, Azliyati.

In: International Journal of Infectious Diseases, Vol. 54, 01.01.2017, p. 85-88.

Research output: Contribution to journalArticle

@article{785a4467bdab4897af4334c1e8a3217d,
title = "Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women",
abstract = "Objectives Human papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. Methods Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. Results HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79{\%} of the samples had normal cytology, while 13{\%} had CIN grade I, 5{\%} had CIN grade II, and 3{\%} had CIN grade III. The analysis revealed that 12{\%} of the samples had CIN cytology and presence of HPV. Approximately 31{\%} had HPV without cervical dysplasia, while 8{\%} of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. Conclusions Overall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.",
keywords = "cervical cancer, CIN, HPV, Kazakhstan, prevalence",
author = "Luiza Niyazmetova and Gulzhanat Aimagambetova and Nazigul Stambekova and Zaurech Abugalieva and Korlukain Seksembayeva and Syed Ali and Azliyati Azizan",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.ijid.2016.11.410",
language = "English",
volume = "54",
pages = "85--88",
journal = "International Journal of Infectious Diseases",
issn = "1201-9712",
publisher = "Elsevier",

}

TY - JOUR

T1 - Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women

AU - Niyazmetova, Luiza

AU - Aimagambetova, Gulzhanat

AU - Stambekova, Nazigul

AU - Abugalieva, Zaurech

AU - Seksembayeva, Korlukain

AU - Ali, Syed

AU - Azizan, Azliyati

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Objectives Human papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. Methods Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. Results HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. Conclusions Overall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.

AB - Objectives Human papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. Methods Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. Results HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. Conclusions Overall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.

KW - cervical cancer

KW - CIN

KW - HPV

KW - Kazakhstan

KW - prevalence

UR - http://www.scopus.com/inward/record.url?scp=85006306870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006306870&partnerID=8YFLogxK

U2 - 10.1016/j.ijid.2016.11.410

DO - 10.1016/j.ijid.2016.11.410

M3 - Article

VL - 54

SP - 85

EP - 88

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

SN - 1201-9712

ER -