Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women

Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova , Zaurech Abugalieva , Korlukain Seksembayeva , Syed Ali , Azliyati Azizan

Research output: Contribution to journalArticle

Abstract

Objectives: Human papillomavirus is themain causative agent for cervical cancer. However, few data are
available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap
smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and
to analyze the association between HPV positivity and the cytology results of patient samples.
Methods: Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient
gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was
done using real-time multiplex PCR.
Results: HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and
18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN
grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had
CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of
samples were CIN positive without HPV infection. A statistically significant relationship between HPV
16 and HPV 33 positive samples and CIN grade II and III was found.
Conclusions: Overall, this study will help to strengthen and guide health policy implementation of
primary and secondary cervical cancer prevention strategies in Kazakhstan.
2016 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-
nd/4.0/).
Original languageEnglish
Pages (from-to)85
Number of pages88
JournalInternational Journal of Infectious Diseases
Volume54
DOIs
Publication statusPublished - Jan 2017

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Kazakhstan
Papanicolaou Test
Cell Biology
Licensure
Uterine Cervical Neoplasms
Human papillomavirus 31
Uterine Cervical Dysplasia
Multiplex Polymerase Chain Reaction
Health Policy
Real-Time Polymerase Chain Reaction
Genotype
DNA
Infection

Cite this

Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova , Zaurech Abugalieva , Korlukain Seksembayeva , Syed Ali , Azliyati Azizan (2017). Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women. International Journal of Infectious Diseases, 54, 85. https://doi.org/doi: 10.1016/j.ijid.2016.11.410. Epub 2016 Nov 25.

Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women. / Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova , Zaurech Abugalieva , Korlukain Seksembayeva , Syed Ali , Azliyati Azizan .

In: International Journal of Infectious Diseases, Vol. 54, 01.2017, p. 85.

Research output: Contribution to journalArticle

Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova , Zaurech Abugalieva , Korlukain Seksembayeva , Syed Ali , Azliyati Azizan 2017, 'Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women', International Journal of Infectious Diseases, vol. 54, pp. 85. https://doi.org/doi: 10.1016/j.ijid.2016.11.410. Epub 2016 Nov 25.
Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova , Zaurech Abugalieva , Korlukain Seksembayeva , Syed Ali , Azliyati Azizan . Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women. International Journal of Infectious Diseases. 2017 Jan;54:85. https://doi.org/doi: 10.1016/j.ijid.2016.11.410. Epub 2016 Nov 25.
Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova , Zaurech Abugalieva , Korlukain Seksembayeva , Syed Ali , Azliyati Azizan . / Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women. In: International Journal of Infectious Diseases. 2017 ; Vol. 54. pp. 85.
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abstract = "Objectives: Human papillomavirus is themain causative agent for cervical cancer. However, few data areavailable about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Papsmear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan andto analyze the association between HPV positivity and the cytology results of patient samples.Methods: Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatientgynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping wasdone using real-time multiplex PCR.Results: HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and18. Microscopic examination showed that 79{\%} of the samples had normal cytology, while 13{\%} had CINgrade I, 5{\%} had CIN grade II, and 3{\%} had CIN grade III. The analysis revealed that 12{\%} of the samples hadCIN cytology and presence of HPV. Approximately 31{\%} had HPV without cervical dysplasia, while 8{\%} ofsamples were CIN positive without HPV infection. A statistically significant relationship between HPV16 and HPV 33 positive samples and CIN grade II and III was found.Conclusions: Overall, this study will help to strengthen and guide health policy implementation ofprimary and secondary cervical cancer prevention strategies in Kazakhstan. 2016 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).",
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