Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry

I. A. Vorobjev, E. P. Rafalovskaya-Orlovskaya, A. A. Gladkih, D. M. Potashnikova, N. S. Barteneva

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Quantum dots (QD) nanocrystals consisting of CdSe core with ZnS shell are a novel class of fluorophores with tremendous potential in microscopy and cytometry techniques. The unique optical features of Qdots, namely, high photostability and extinction coefficient, wide absorption and narrow emission spectra, and large Stokes shift make them desirable fluorescent tags for diverse biomedical applications. Applications of this novel technology in microscopy and cytometry produce reliable multicolor specimens due to increased photostability, ability for multiplexing and narrow emission spectra of nanocrystals. QD conjugates are available on the market and could be prepared in the laboratory. This paper describes the application of QD-conjugates for immunophenotyping and FISH assessment of cells and tissues, and the requirements for microscope and flow cytometer reengineering for successful use of QD in multiplex fluorescent format. Despite the considerable progress, important methodological issues still need to be solved in terms of QD nanocrystals' size, heterogeneity, functionalization and stability of their conjugates. We discuss practical approaches and challenges that need to be addressed to make QD immunostaining a standard method in biology.

Original languageEnglish
Pages (from-to)392-403
Number of pages12
JournalTsitologiya
Volume53
Issue number5
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Quantum Dots
Microscopy
Nanoparticles
Immunophenotyping
Technology

Keywords

  • Flow cytometry
  • Fluorescent nanocrystals
  • Immunohistochemistry
  • Quantum dots

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Vorobjev, I. A., Rafalovskaya-Orlovskaya, E. P., Gladkih, A. A., Potashnikova, D. M., & Barteneva, N. S. (2011). Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry. Tsitologiya, 53(5), 392-403.

Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry. / Vorobjev, I. A.; Rafalovskaya-Orlovskaya, E. P.; Gladkih, A. A.; Potashnikova, D. M.; Barteneva, N. S.

In: Tsitologiya, Vol. 53, No. 5, 2011, p. 392-403.

Research output: Contribution to journalArticle

Vorobjev, IA, Rafalovskaya-Orlovskaya, EP, Gladkih, AA, Potashnikova, DM & Barteneva, NS 2011, 'Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry', Tsitologiya, vol. 53, no. 5, pp. 392-403.
Vorobjev IA, Rafalovskaya-Orlovskaya EP, Gladkih AA, Potashnikova DM, Barteneva NS. Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry. Tsitologiya. 2011;53(5):392-403.
Vorobjev, I. A. ; Rafalovskaya-Orlovskaya, E. P. ; Gladkih, A. A. ; Potashnikova, D. M. ; Barteneva, N. S. / Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry. In: Tsitologiya. 2011 ; Vol. 53, No. 5. pp. 392-403.
@article{91f17428e2074788ab586794a1197a88,
title = "Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry",
abstract = "Quantum dots (QD) nanocrystals consisting of CdSe core with ZnS shell are a novel class of fluorophores with tremendous potential in microscopy and cytometry techniques. The unique optical features of Qdots, namely, high photostability and extinction coefficient, wide absorption and narrow emission spectra, and large Stokes shift make them desirable fluorescent tags for diverse biomedical applications. Applications of this novel technology in microscopy and cytometry produce reliable multicolor specimens due to increased photostability, ability for multiplexing and narrow emission spectra of nanocrystals. QD conjugates are available on the market and could be prepared in the laboratory. This paper describes the application of QD-conjugates for immunophenotyping and FISH assessment of cells and tissues, and the requirements for microscope and flow cytometer reengineering for successful use of QD in multiplex fluorescent format. Despite the considerable progress, important methodological issues still need to be solved in terms of QD nanocrystals' size, heterogeneity, functionalization and stability of their conjugates. We discuss practical approaches and challenges that need to be addressed to make QD immunostaining a standard method in biology.",
keywords = "Flow cytometry, Fluorescent nanocrystals, Immunohistochemistry, Quantum dots",
author = "Vorobjev, {I. A.} and Rafalovskaya-Orlovskaya, {E. P.} and Gladkih, {A. A.} and Potashnikova, {D. M.} and Barteneva, {N. S.}",
year = "2011",
language = "English",
volume = "53",
pages = "392--403",
journal = "Tsitologiya",
issn = "0041-3771",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "5",

}

TY - JOUR

T1 - Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry

AU - Vorobjev, I. A.

AU - Rafalovskaya-Orlovskaya, E. P.

AU - Gladkih, A. A.

AU - Potashnikova, D. M.

AU - Barteneva, N. S.

PY - 2011

Y1 - 2011

N2 - Quantum dots (QD) nanocrystals consisting of CdSe core with ZnS shell are a novel class of fluorophores with tremendous potential in microscopy and cytometry techniques. The unique optical features of Qdots, namely, high photostability and extinction coefficient, wide absorption and narrow emission spectra, and large Stokes shift make them desirable fluorescent tags for diverse biomedical applications. Applications of this novel technology in microscopy and cytometry produce reliable multicolor specimens due to increased photostability, ability for multiplexing and narrow emission spectra of nanocrystals. QD conjugates are available on the market and could be prepared in the laboratory. This paper describes the application of QD-conjugates for immunophenotyping and FISH assessment of cells and tissues, and the requirements for microscope and flow cytometer reengineering for successful use of QD in multiplex fluorescent format. Despite the considerable progress, important methodological issues still need to be solved in terms of QD nanocrystals' size, heterogeneity, functionalization and stability of their conjugates. We discuss practical approaches and challenges that need to be addressed to make QD immunostaining a standard method in biology.

AB - Quantum dots (QD) nanocrystals consisting of CdSe core with ZnS shell are a novel class of fluorophores with tremendous potential in microscopy and cytometry techniques. The unique optical features of Qdots, namely, high photostability and extinction coefficient, wide absorption and narrow emission spectra, and large Stokes shift make them desirable fluorescent tags for diverse biomedical applications. Applications of this novel technology in microscopy and cytometry produce reliable multicolor specimens due to increased photostability, ability for multiplexing and narrow emission spectra of nanocrystals. QD conjugates are available on the market and could be prepared in the laboratory. This paper describes the application of QD-conjugates for immunophenotyping and FISH assessment of cells and tissues, and the requirements for microscope and flow cytometer reengineering for successful use of QD in multiplex fluorescent format. Despite the considerable progress, important methodological issues still need to be solved in terms of QD nanocrystals' size, heterogeneity, functionalization and stability of their conjugates. We discuss practical approaches and challenges that need to be addressed to make QD immunostaining a standard method in biology.

KW - Flow cytometry

KW - Fluorescent nanocrystals

KW - Immunohistochemistry

KW - Quantum dots

UR - http://www.scopus.com/inward/record.url?scp=79961216194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961216194&partnerID=8YFLogxK

M3 - Article

C2 - 21786682

AN - SCOPUS:79961216194

VL - 53

SP - 392

EP - 403

JO - Tsitologiya

JF - Tsitologiya

SN - 0041-3771

IS - 5

ER -