Aptamer-based electrochemical biosensor for interferon gamma detection

Ying Liu, Nazgul Tuleouva, Erlan Ramanculov, Alexander Revzin

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.

Original languageEnglish
Pages (from-to)8131-8136
Number of pages6
JournalAnalytical Chemistry
Volume82
Issue number19
DOIs
Publication statusPublished - Oct 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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  • Cite this

    Liu, Y., Tuleouva, N., Ramanculov, E., & Revzin, A. (2010). Aptamer-based electrochemical biosensor for interferon gamma detection. Analytical Chemistry, 82(19), 8131-8136. https://doi.org/10.1021/ac101409t