Assembly of tight junction is regulated by the antagonism of conventional and novel protein kinase C isoforms

Anna Y. Andreeva, Jörg Piontek, Ingolf E. Blasig, Darkhan I. Utepbergenov

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Apparently conflicting observations indicated that protein kinase C both may block and support the assembly of tight junctions. We therefore tested the hypothesis that different isoenzymes antagonistically affect tight junction proteins and function. Thus, by using specific inhibitors we investigated the involvement of conventional and novel protein kinase C of kidney tubule cells in tight junction assembly. In low Ca2+ medium, the application of pan-protein kinase C inhibitor GF-109203X blocked the formation of tight junctions induced by protein kinase C agonist diacyglycerol. Gö6976, inhibitor of conventional protein kinase C, promoted the formation of tight junctions and occludin phosphorylation in cells cultivated in low Ca 2+ medium and attenuated the disruption of tight junction complex induced by the switch to low Ca2+ medium. In addition, Gö6976 accelerated the occludin phosphorylation and the formation of tight junction barrier during assembly of tight junctions induced by Ca2+ re-addition. This phosphorylation was accompanied by accelerated occludin incorporation into newly forming tight junctions and by reducing the paracellular permeability. In contrast, inhibitor of novel protein kinase C rottlerin blocked the occludin phosphorylation and the formation of tight junction barrier, both caused by re-addition of normal Ca2+ medium. It is concluded that the conventional protein kinase C alpha participates in tight junction disassembly while the novel protein kinase C epsilon plays a role in tight junction formation of kidney epithelial cells. The discovered antagonism contributes to a better understanding of the regulation of the structure and function of tight junctions and hence to that of the epithelial barrier.

Original languageEnglish
Pages (from-to)222-233
Number of pages12
JournalInternational Journal of Biochemistry and Cell Biology
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Tight Junctions
Protein Kinase C
Occludin
Protein Isoforms
Phosphorylation
Tight Junction Proteins
Protein Kinase C-epsilon
Protein Kinase C-alpha
Isoenzymes
Switch Genes
Kidney Tubules
Switches
Protein C Inhibitor
Protein Kinase Inhibitors
Permeability
Epithelial Cells
Kidney

Keywords

  • Occludin
  • Paracellular tightness
  • Protein kinase C
  • Tight junctions

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

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title = "Assembly of tight junction is regulated by the antagonism of conventional and novel protein kinase C isoforms",
abstract = "Apparently conflicting observations indicated that protein kinase C both may block and support the assembly of tight junctions. We therefore tested the hypothesis that different isoenzymes antagonistically affect tight junction proteins and function. Thus, by using specific inhibitors we investigated the involvement of conventional and novel protein kinase C of kidney tubule cells in tight junction assembly. In low Ca2+ medium, the application of pan-protein kinase C inhibitor GF-109203X blocked the formation of tight junctions induced by protein kinase C agonist diacyglycerol. G{\"o}6976, inhibitor of conventional protein kinase C, promoted the formation of tight junctions and occludin phosphorylation in cells cultivated in low Ca 2+ medium and attenuated the disruption of tight junction complex induced by the switch to low Ca2+ medium. In addition, G{\"o}6976 accelerated the occludin phosphorylation and the formation of tight junction barrier during assembly of tight junctions induced by Ca2+ re-addition. This phosphorylation was accompanied by accelerated occludin incorporation into newly forming tight junctions and by reducing the paracellular permeability. In contrast, inhibitor of novel protein kinase C rottlerin blocked the occludin phosphorylation and the formation of tight junction barrier, both caused by re-addition of normal Ca2+ medium. It is concluded that the conventional protein kinase C alpha participates in tight junction disassembly while the novel protein kinase C epsilon plays a role in tight junction formation of kidney epithelial cells. The discovered antagonism contributes to a better understanding of the regulation of the structure and function of tight junctions and hence to that of the epithelial barrier.",
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AU - Blasig, Ingolf E.

AU - Utepbergenov, Darkhan I.

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