Assessment of tissue scaffold degradation using electrochemical techniques

Alison Willows, Qiang Fan, Fanya Ismail, Claudia M. Vaz, Paul E. Tomlins, Lyuba I. Mikhalovska, Sergey V. Mikhalovsky, Stuart L. James, Pankaj Vadgama, Jaroslaw Wasikiewicz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Degradation of a commercially available collagen-glycosaminoglycan dermal equivalent matrix was studied using electrochemical techniques. Degradation was accelerated by exposure to gamma radiation followed by storage at elevated temperatures or exposure to enzymes. The time-dependent diffusion of a small, electrochemically active, molecular probe, potassium ferrocyanide, through the matrix was monitored via changes in the oxidation peak currents of cyclic voltammograms. These measurements were made using a two-compartment diffusion chamber with the sample positioned well away from the working electrodes and a single-compartment electrode cell where the matrix was in direct contact with the working electrode. The relative merits of these two approaches are considered. Regardless of the approach chosen, amperometry appears well suited to monitoring progressive diffusivity changes through mechanically weak porous structures subject to different solution environments.

Original languageEnglish
Pages (from-to)686-696
Number of pages11
JournalActa Biomaterialia
Volume4
Issue number3
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Electrochemical Techniques
Tissue Scaffolds
Electrodes
Degradation
Molecular Probes
Gamma Rays
Glycosaminoglycans
Collagen
Gamma rays
Potassium
Enzymes
Oxidation
Skin
Temperature
Monitoring

Keywords

  • Amperometry
  • Degradation
  • Electrochemistry
  • Porous structure
  • Tissue scaffold

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Willows, A., Fan, Q., Ismail, F., Vaz, C. M., Tomlins, P. E., Mikhalovska, L. I., ... Wasikiewicz, J. (2008). Assessment of tissue scaffold degradation using electrochemical techniques. Acta Biomaterialia, 4(3), 686-696. https://doi.org/10.1016/j.actbio.2007.10.009

Assessment of tissue scaffold degradation using electrochemical techniques. / Willows, Alison; Fan, Qiang; Ismail, Fanya; Vaz, Claudia M.; Tomlins, Paul E.; Mikhalovska, Lyuba I.; Mikhalovsky, Sergey V.; James, Stuart L.; Vadgama, Pankaj; Wasikiewicz, Jaroslaw.

In: Acta Biomaterialia, Vol. 4, No. 3, 05.2008, p. 686-696.

Research output: Contribution to journalArticle

Willows, A, Fan, Q, Ismail, F, Vaz, CM, Tomlins, PE, Mikhalovska, LI, Mikhalovsky, SV, James, SL, Vadgama, P & Wasikiewicz, J 2008, 'Assessment of tissue scaffold degradation using electrochemical techniques', Acta Biomaterialia, vol. 4, no. 3, pp. 686-696. https://doi.org/10.1016/j.actbio.2007.10.009
Willows A, Fan Q, Ismail F, Vaz CM, Tomlins PE, Mikhalovska LI et al. Assessment of tissue scaffold degradation using electrochemical techniques. Acta Biomaterialia. 2008 May;4(3):686-696. https://doi.org/10.1016/j.actbio.2007.10.009
Willows, Alison ; Fan, Qiang ; Ismail, Fanya ; Vaz, Claudia M. ; Tomlins, Paul E. ; Mikhalovska, Lyuba I. ; Mikhalovsky, Sergey V. ; James, Stuart L. ; Vadgama, Pankaj ; Wasikiewicz, Jaroslaw. / Assessment of tissue scaffold degradation using electrochemical techniques. In: Acta Biomaterialia. 2008 ; Vol. 4, No. 3. pp. 686-696.
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