TY - JOUR
T1 - Association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment
AU - Ahmed, Muhammad Adeel
AU - Nazim, Fizza
AU - Ahmed, Khalid
AU - Bari, Muhammad Furqan
AU - Abdulwahed, Abdulaziz
AU - AlMokhatieb, Ahmed A
AU - Alalvi, Yaseen
AU - Abduljabbar, Tariq
AU - Mughal, Nouman
AU - Abidi, Syed Hani
N1 - © 2022. The Author(s).
PY - 2022/8/15
Y1 - 2022/8/15
N2 - In this study, we have investigated the association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment. Twenty-seven patients aged between 15 and 57 years underwent periapical surgery. The retrieved periapical tissue sample was used for mRNA expression analysis of COL1A1, VTN, ITGA5, IL-4, TNF, ANGPT, VEGFA, and CTGF. All patients were recalled after 6 and 12 months for periapical healing evaluation. Healing was then correlated with baseline gene expression. Healing was observed in 15 patients at the end of 6 months, which increased to 21 patients after 12 months. Six patients showed no healing even after 12 months. Analysis of baseline expression levels of the tested genes with healing status showed the mean relative expression of VTN, VEGFA, ANGPT, TNF, and CTGF to be significantly different (p < 0.05) between the healing group (6 and 12 months) (72.99%) and the non-healing (94.42%) group. Periapical Index scores 3-5 exhibited a positive correlation with ITGA-5 expression. Overexpression of ANGPT and a strong positive correlation between ITGA5 and PAI scores in the non-healing group of patients may suggest these genes to be a potential prognostic biomarker for periapical wound non-healing after surgical endodontic treatment.
AB - In this study, we have investigated the association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment. Twenty-seven patients aged between 15 and 57 years underwent periapical surgery. The retrieved periapical tissue sample was used for mRNA expression analysis of COL1A1, VTN, ITGA5, IL-4, TNF, ANGPT, VEGFA, and CTGF. All patients were recalled after 6 and 12 months for periapical healing evaluation. Healing was then correlated with baseline gene expression. Healing was observed in 15 patients at the end of 6 months, which increased to 21 patients after 12 months. Six patients showed no healing even after 12 months. Analysis of baseline expression levels of the tested genes with healing status showed the mean relative expression of VTN, VEGFA, ANGPT, TNF, and CTGF to be significantly different (p < 0.05) between the healing group (6 and 12 months) (72.99%) and the non-healing (94.42%) group. Periapical Index scores 3-5 exhibited a positive correlation with ITGA-5 expression. Overexpression of ANGPT and a strong positive correlation between ITGA5 and PAI scores in the non-healing group of patients may suggest these genes to be a potential prognostic biomarker for periapical wound non-healing after surgical endodontic treatment.
KW - Adolescent
KW - Adult
KW - Humans
KW - Middle Aged
KW - Periapical Granuloma/genetics
KW - Periapical Periodontitis
KW - Transcriptome
KW - Wound Healing/genetics
KW - Young Adult
U2 - 10.1038/s41598-022-17774-z
DO - 10.1038/s41598-022-17774-z
M3 - Article
C2 - 35970906
SN - 2045-2322
VL - 12
SP - 13824
JO - Scientific Reports
JF - Scientific Reports
IS - 1
ER -
