Automated cell counting in tissue sections: a new approach by ‘multiple grey‐level analysis‘

D. Wynford‐Thomas, N. Garrahan, B. Jasani, E. D. Williams

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3 Citations (Scopus)

Abstract

A system is described for the automated quantitation in tissue sections of the follicular and stromal cell populations of the thyroid gland, using a computer‐linked TV image analyser. The paper illustrates how the problems of variable staining intensity and clustering of images (nuclei) may be overcome by a novel approach, in which multiple analyses of each field are made at increasing grey‐level thresholds, and then ‘synthesized’ by computer to give a composite image. Discrimination between cell (nuclear) types was by a single minimum width criterion. The results were highly reproducible and correlated well with counts obtained by a comparable manual method. 1982 Blackwell Science Ltd

Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalJournal of Microscopy
Volume127
Issue number2
DOIs
Publication statusPublished - Aug 1982

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Keywords

  • Image analysis
  • cell counting
  • cluster resolution
  • thyroid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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