Automated cell counting in tissue sections

A new approach by 'multiple grey-level analysis'

D. Wynford-Thomas, N. Garrahan, B. Jasani, E. D. Williams

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A system is described for the automated quantitation in tissue sections of the follicular and stromal cell populations of the thyroid gland, using a computer-linked TV image analyser. The paper illustrates how the problems of variable staining intensity and clustering of images (nuclei) may be overcome by a novel approach, in which multiple analysis of each field are made at increasing grey-level thresholds, and then 'synthesized' by computer to give a composite image. Discrimination between cell (nuclear) types was by a single minimum width criterion. The results were highly reproducible and correlated well with counts obtained by a comparable manual method.

Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalJournal of Microscopy
Volume127
Issue number2
Publication statusPublished - 1982
Externally publishedYes

Fingerprint

counting
Tissue
Stromal Cells
cells
thyroid gland
Cluster Analysis
Thyroid Gland
Cells
staining
Staining and Labeling
discrimination
Composite materials
Population
nuclei
composite materials
thresholds

ASJC Scopus subject areas

  • Instrumentation

Cite this

Wynford-Thomas, D., Garrahan, N., Jasani, B., & Williams, E. D. (1982). Automated cell counting in tissue sections: A new approach by 'multiple grey-level analysis'. Journal of Microscopy, 127(2), 175-184.

Automated cell counting in tissue sections : A new approach by 'multiple grey-level analysis'. / Wynford-Thomas, D.; Garrahan, N.; Jasani, B.; Williams, E. D.

In: Journal of Microscopy, Vol. 127, No. 2, 1982, p. 175-184.

Research output: Contribution to journalArticle

Wynford-Thomas, D, Garrahan, N, Jasani, B & Williams, ED 1982, 'Automated cell counting in tissue sections: A new approach by 'multiple grey-level analysis'', Journal of Microscopy, vol. 127, no. 2, pp. 175-184.
Wynford-Thomas D, Garrahan N, Jasani B, Williams ED. Automated cell counting in tissue sections: A new approach by 'multiple grey-level analysis'. Journal of Microscopy. 1982;127(2):175-184.
Wynford-Thomas, D. ; Garrahan, N. ; Jasani, B. ; Williams, E. D. / Automated cell counting in tissue sections : A new approach by 'multiple grey-level analysis'. In: Journal of Microscopy. 1982 ; Vol. 127, No. 2. pp. 175-184.
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