Beta-amyloid peptides stimulate endozepine biosynthesis in cultured rat astrocytes

Tursonjan Tokay, Olfa Masmoudi, Pierrick Gandolfo, Jérôme Leprince, Georges Pelletier, Hubert Vaudry, Marie Christine Tonon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Accumulation of β-amyloid peptide (Aβ), which is a landmark of Alzheimer's disease, may alter astrocyte functions before any visible symptoms of the disease occur. Here, we examined the effects of Aβ on biosynthesis and release of diazepam-binding inhibitor (DBI), a polypeptide primarily expressed by astroglial cells in the CNS. Quantitative RT-PCR and specific radioimmunoassay demonstrated that aggregated Aβ25-35, at concentrations up to 10-4 M, induced a dose-dependent increase in DBI mRNA expression and DBI-related peptide release from cultured rat astrocytes. These effects were totally suppressed when aggregation of Aβ 25-35 was prevented by Congo red. Measurement of the number of living cells revealed that Aβ25-35 induced a trophic rather than a toxic effect on astrocytes. Administration of cycloheximide blocked Aβ25-35-induced increase of DBI gene expression and endozepine accumulation in astrocytes, indicating that protein synthesis is required for DBI gene expression. Altogether, the present data suggest that Aβ-induced activation of endozepine biosynthesis and release may contribute to astrocyte proliferation associated with Alzheimer's disease.

Original languageEnglish
Pages (from-to)607-616
Number of pages10
JournalJournal of Neurochemistry
Volume94
Issue number3
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Diazepam Binding Inhibitor
Amyloid beta-Peptides
Biosynthesis
Astrocytes
Rats
Gene expression
Alzheimer Disease
Gene Expression
Congo Red
Peptides
Poisons
Cycloheximide
Amyloid
Radioimmunoassay
Agglomeration
Cell Count
Chemical activation
Cells
Polymerase Chain Reaction
Messenger RNA

Keywords

  • β-amyloid protein
  • Astrocytes
  • Diazepam-binding inhibitor
  • Octadecaneuropeptide

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Tokay, T., Masmoudi, O., Gandolfo, P., Leprince, J., Pelletier, G., Vaudry, H., & Tonon, M. C. (2005). Beta-amyloid peptides stimulate endozepine biosynthesis in cultured rat astrocytes. Journal of Neurochemistry, 94(3), 607-616. https://doi.org/10.1111/j.1471-4159.2005.03102.x

Beta-amyloid peptides stimulate endozepine biosynthesis in cultured rat astrocytes. / Tokay, Tursonjan; Masmoudi, Olfa; Gandolfo, Pierrick; Leprince, Jérôme; Pelletier, Georges; Vaudry, Hubert; Tonon, Marie Christine.

In: Journal of Neurochemistry, Vol. 94, No. 3, 08.2005, p. 607-616.

Research output: Contribution to journalArticle

Tokay, T, Masmoudi, O, Gandolfo, P, Leprince, J, Pelletier, G, Vaudry, H & Tonon, MC 2005, 'Beta-amyloid peptides stimulate endozepine biosynthesis in cultured rat astrocytes', Journal of Neurochemistry, vol. 94, no. 3, pp. 607-616. https://doi.org/10.1111/j.1471-4159.2005.03102.x
Tokay, Tursonjan ; Masmoudi, Olfa ; Gandolfo, Pierrick ; Leprince, Jérôme ; Pelletier, Georges ; Vaudry, Hubert ; Tonon, Marie Christine. / Beta-amyloid peptides stimulate endozepine biosynthesis in cultured rat astrocytes. In: Journal of Neurochemistry. 2005 ; Vol. 94, No. 3. pp. 607-616.
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