Biochemical analysis and subcellular distribution of E-cadherin-catenin in adenocarcinomas of the gastro-oesophageal junction

B. P.L. Wijnhoven, E. T. Tucker, W. N.M. Dinjens, H. W. Tilanus, M. Pignatelli

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Abstract

Background: Disturbances in the expression or structure of E-cadherin-catenin, a cell-cell adhesion complex, perturb its cell adhesive function. Materials and Methods: We studied the expression and distribution of the E-cadherin-catenin complex in 24 adenocarcinomas of the gastro-oesophageal junction (GOJ) by immunohistochemistry and Western blotting of the Triton X-100-soluble (membrane bound) and insoluble fractions (cytoskeleton bound). Results: Immunohistochemistry demonstrated redistribution of E-cadherin, α-, β- and γ-catenin from the membrane to the cytoplasm in 13/24 (54%), 18/24 (75%), 16/24 (67%) and 15/24 (63%) tumours, respectively. Five tumours showed nuclear localisation of β-catenin. Western blotting showed redistribution between the TX-100 soluble and insoluble fraction of E-cadherin and the catenins in 5/11 (45%), 4/10 (40%), 5/11 (45%) and 5/11 (45%) tumours, respectively. Conclusion: Loss of membrane bound E-cadherin-catenin is frequently observed in adenocarcinomas of the GOJ and this may reflect loss of function of the E-cadherin-catenin complex in these cancers.

Original languageEnglish
Pages (from-to)1369-1375
Number of pages7
JournalAnticancer Research
Volume24
Issue number3 A
Publication statusPublished - May 1 2004

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Keywords

  • Adenocarcinoma
  • E-cadherin-catenin
  • Gastrooesophageal junction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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