Biochemical and functional characterization of the murine ros protooncogene

Dieter Riethmacher, Oliver Langholz, Steffi Gödecke, Martin Sachs, Carmen Birchmeier

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The ros gene was originally found because it can, when mutated, induce malignant transformation. The protooncogene encodes an orphan receptor tyrosine kinase. We report here the isolation and characterization of the mouse c-ros cDNA and, in addition, the biochemical characterization of the receptor. Both, the endogenous c-ros protein from embryonal tissues and the recombinant protein are glycosylated molecules with an apparent molecular weight of 260 000. Pulse-chase analysis in Sf9 cells demonstrates that the c-ros protein is synthesized as a single chain, uncleaved molecule. Since the specific ligand of c-ros is not known, a hybrid receptor (trk/c-ros) which transmits c-ros-specific signals in response to nerve growth factor (NGF) was used to study the biological activities. In NIH3T3 cells, this trk/c-ros hybrid induces growth, a fusiform cell shape, and loss of contact inhibition of growth. However, the active hybrid receptor cannot replace IL-3 as survival factor in 32D myeloid cells. Compared to other receptors, the active c-ros tyrosine kinase domain displays thus overlapping, but not identical signalling specificities.

Original languageEnglish
Pages (from-to)3617-3626
Number of pages10
JournalOncogene
Volume9
Issue number12
Publication statusPublished - Dec 1994
Externally publishedYes

Fingerprint

Contact Inhibition
Sf9 Cells
Cell Shape
Interleukin-3
Receptor Protein-Tyrosine Kinases
Nerve Growth Factor
Myeloid Cells
Growth
Recombinant Proteins
Protein-Tyrosine Kinases
Proteins
Complementary DNA
Molecular Weight
Ligands
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Riethmacher, D., Langholz, O., Gödecke, S., Sachs, M., & Birchmeier, C. (1994). Biochemical and functional characterization of the murine ros protooncogene. Oncogene, 9(12), 3617-3626.

Biochemical and functional characterization of the murine ros protooncogene. / Riethmacher, Dieter; Langholz, Oliver; Gödecke, Steffi; Sachs, Martin; Birchmeier, Carmen.

In: Oncogene, Vol. 9, No. 12, 12.1994, p. 3617-3626.

Research output: Contribution to journalArticle

Riethmacher, D, Langholz, O, Gödecke, S, Sachs, M & Birchmeier, C 1994, 'Biochemical and functional characterization of the murine ros protooncogene', Oncogene, vol. 9, no. 12, pp. 3617-3626.
Riethmacher D, Langholz O, Gödecke S, Sachs M, Birchmeier C. Biochemical and functional characterization of the murine ros protooncogene. Oncogene. 1994 Dec;9(12):3617-3626.
Riethmacher, Dieter ; Langholz, Oliver ; Gödecke, Steffi ; Sachs, Martin ; Birchmeier, Carmen. / Biochemical and functional characterization of the murine ros protooncogene. In: Oncogene. 1994 ; Vol. 9, No. 12. pp. 3617-3626.
@article{44333ac511d7408a87b9e6966f7fa9e0,
title = "Biochemical and functional characterization of the murine ros protooncogene",
abstract = "The ros gene was originally found because it can, when mutated, induce malignant transformation. The protooncogene encodes an orphan receptor tyrosine kinase. We report here the isolation and characterization of the mouse c-ros cDNA and, in addition, the biochemical characterization of the receptor. Both, the endogenous c-ros protein from embryonal tissues and the recombinant protein are glycosylated molecules with an apparent molecular weight of 260 000. Pulse-chase analysis in Sf9 cells demonstrates that the c-ros protein is synthesized as a single chain, uncleaved molecule. Since the specific ligand of c-ros is not known, a hybrid receptor (trk/c-ros) which transmits c-ros-specific signals in response to nerve growth factor (NGF) was used to study the biological activities. In NIH3T3 cells, this trk/c-ros hybrid induces growth, a fusiform cell shape, and loss of contact inhibition of growth. However, the active hybrid receptor cannot replace IL-3 as survival factor in 32D myeloid cells. Compared to other receptors, the active c-ros tyrosine kinase domain displays thus overlapping, but not identical signalling specificities.",
author = "Dieter Riethmacher and Oliver Langholz and Steffi G{\"o}decke and Martin Sachs and Carmen Birchmeier",
year = "1994",
month = "12",
language = "English",
volume = "9",
pages = "3617--3626",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Biochemical and functional characterization of the murine ros protooncogene

AU - Riethmacher, Dieter

AU - Langholz, Oliver

AU - Gödecke, Steffi

AU - Sachs, Martin

AU - Birchmeier, Carmen

PY - 1994/12

Y1 - 1994/12

N2 - The ros gene was originally found because it can, when mutated, induce malignant transformation. The protooncogene encodes an orphan receptor tyrosine kinase. We report here the isolation and characterization of the mouse c-ros cDNA and, in addition, the biochemical characterization of the receptor. Both, the endogenous c-ros protein from embryonal tissues and the recombinant protein are glycosylated molecules with an apparent molecular weight of 260 000. Pulse-chase analysis in Sf9 cells demonstrates that the c-ros protein is synthesized as a single chain, uncleaved molecule. Since the specific ligand of c-ros is not known, a hybrid receptor (trk/c-ros) which transmits c-ros-specific signals in response to nerve growth factor (NGF) was used to study the biological activities. In NIH3T3 cells, this trk/c-ros hybrid induces growth, a fusiform cell shape, and loss of contact inhibition of growth. However, the active hybrid receptor cannot replace IL-3 as survival factor in 32D myeloid cells. Compared to other receptors, the active c-ros tyrosine kinase domain displays thus overlapping, but not identical signalling specificities.

AB - The ros gene was originally found because it can, when mutated, induce malignant transformation. The protooncogene encodes an orphan receptor tyrosine kinase. We report here the isolation and characterization of the mouse c-ros cDNA and, in addition, the biochemical characterization of the receptor. Both, the endogenous c-ros protein from embryonal tissues and the recombinant protein are glycosylated molecules with an apparent molecular weight of 260 000. Pulse-chase analysis in Sf9 cells demonstrates that the c-ros protein is synthesized as a single chain, uncleaved molecule. Since the specific ligand of c-ros is not known, a hybrid receptor (trk/c-ros) which transmits c-ros-specific signals in response to nerve growth factor (NGF) was used to study the biological activities. In NIH3T3 cells, this trk/c-ros hybrid induces growth, a fusiform cell shape, and loss of contact inhibition of growth. However, the active hybrid receptor cannot replace IL-3 as survival factor in 32D myeloid cells. Compared to other receptors, the active c-ros tyrosine kinase domain displays thus overlapping, but not identical signalling specificities.

UR - http://www.scopus.com/inward/record.url?scp=0028037189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028037189&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 3617

EP - 3626

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 12

ER -