Abstract
The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications.
| Original language | English |
|---|---|
| Pages (from-to) | 324-333 |
| Number of pages | 10 |
| Journal | International Journal of Biological Macromolecules |
| Volume | 114 |
| DOIs | |
| Publication status | Published - Jul 15 2018 |
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Keywords
- 1,1,3,3-tetramethoxypropane
- Chitosan
- Cryogels
- Natural polymers
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Molecular Biology
Cite this
Biocompatible scaffolds based on natural polymers for regenerative medicine. / Akilbekova, Dana; Shaimerdenova, Madina; Adilov, Salimgerey; Berillo, Dmitriy.
In: International Journal of Biological Macromolecules, Vol. 114, 15.07.2018, p. 324-333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biocompatible scaffolds based on natural polymers for regenerative medicine
AU - Akilbekova, Dana
AU - Shaimerdenova, Madina
AU - Adilov, Salimgerey
AU - Berillo, Dmitriy
PY - 2018/7/15
Y1 - 2018/7/15
N2 - The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications.
AB - The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications.
KW - 1,1,3,3-tetramethoxypropane
KW - Chitosan
KW - Cryogels
KW - Natural polymers
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UR - http://www.scopus.com/inward/citedby.url?scp=85044583948&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2018.03.116
DO - 10.1016/j.ijbiomac.2018.03.116
M3 - Article
VL - 114
SP - 324
EP - 333
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -