Bioinspired detoxification of blood

The efficient removal of anthrax toxin protective antigen using an extracorporeal macroporous adsorbent devicearticle

Ganesh Ingavle, Les Baillie, Nathan Davies, Nigel Beaton, Yishan Zheng, Sergey Mikhalovsky, Susan Sandeman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Whilst various remedial human monoclonal antibodies have been developed to treat the potentially life-threatening systemic complications associated with anthrax infection, an optimal and universally effective administration route has yet to be established. In the later stages of infection when antibody administration by injection is more likely to fail one possible route to improve outcome is via the use of an antibody-bound, adsorbent haemoperfusion device. We report here the development of an adsorbent macroporous polymer column containing immobilised B. anthracis exotoxin-specific antibodies, PANG (a non-glycosylated, version of a plant-produced human monoclonal antibody) and Valortim (a fully human monoclonal N-linked glycosylated antibody), for removal of anthrax protective antigen (PA) from freshly frozen human plasma and human whole blood. In addition, we have demonstrated that continuous extracorporeal blood recirculation through a Valortim-bound haemoperfusion column significantly reduced the blood plasma concentration of anthrax PA over 2 hours using an in vivo PA rat infusion model. This work provides proof-of-concept evidence to support the development of such alternative detoxification platforms.

Original languageEnglish
Article number7518
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Hemoperfusion
Antibodies
Monoclonal Antibodies
Exotoxins
Anthrax
Infection
Polymers
anthrax toxin
Antigens
Equipment and Supplies
Injections

ASJC Scopus subject areas

  • General

Cite this

Bioinspired detoxification of blood : The efficient removal of anthrax toxin protective antigen using an extracorporeal macroporous adsorbent devicearticle. / Ingavle, Ganesh; Baillie, Les; Davies, Nathan; Beaton, Nigel; Zheng, Yishan; Mikhalovsky, Sergey; Sandeman, Susan.

In: Scientific Reports, Vol. 8, No. 1, 7518, 01.12.2018.

Research output: Contribution to journalArticle

Ingavle, Ganesh ; Baillie, Les ; Davies, Nathan ; Beaton, Nigel ; Zheng, Yishan ; Mikhalovsky, Sergey ; Sandeman, Susan. / Bioinspired detoxification of blood : The efficient removal of anthrax toxin protective antigen using an extracorporeal macroporous adsorbent devicearticle. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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