Biological and polymeric self-assembled hybrid systems: Structure and properties of thylakoid/polyelectrolyte complexes

A. A. Dementiev, A. A. Baikov, V. V. Ptushenko, G. B. Khomutov, A. N. Tikhonov

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A novel hybrid system composed of biological components and synthetic polymer, thylakoid/polycation complex, has been formed and studied. Effects of complex formation on the structure, electrostatics and functioning of thylakoid membranes have been examined. Thylakoids from bean leaves were used to form complexes with polycation polyallylamine hydrochloride (PAAH) in two systems: (i) thylakoid/polycation complexes formed in an aqueous bulk phase, and (ii) immobilized thylakoid/polycation planar complexes. Immobilized on a solid substrate surface, thylakoid/polycation complexes were prepared using layer-by-layer stepwise alternate adsorption technique, i.e., via the sequential alternate adsorption of thylakoids and polycation molecules. The morphology of built up structures was investigated by scanning electron microscopy. Light-induced electron transport in chloroplasts was studied by the electron paramagnetic resonance (EPR) method. Spin probe technique was employed to study the structural and electrostatic characteristics of thylakoid membranes. We have found that efficiency of light-induced electron transport in thylakoid membranes and membrane structure were not changed noticeably by PAAH binding to thylakoids in a wide range of PAAH concentrations. The data obtained indicate the physiologically-soft character of polycation interactions with thylakoid membranes and demonstrate effectiveness of interfacial self-assembly approach to fabrication of complex planar functional nanostructures from biological components and synthetic polymers.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1712
Issue number1
DOIs
Publication statusPublished - Jun 15 2005
Externally publishedYes

Keywords

  • EPR
  • Electron transport
  • Layer-by-layer deposition
  • Polyelectrolytes
  • Thylakoid membranes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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