Boronate-containing polymer brushes

Characterization, interaction with saccharides and mammalian cancer cells

Alexander E. Ivanov, John Eccles, Homayon Ahmad Panahi, Ashok Kumar, Marina V. Kuzimenkova, Lars Nilsson, Björn Bergenståhl, Nick Long, Gary J. Phillips, Sergey V. Mikhalovsky, Igor Yu Galaev, Bo Mattiasson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Boronate-containing polymer brushes were synthesized by free radical copolymerization of N,N-dimethylacrylamide (DMAA) and N-acryloyl-m-phenylboronic acid (NAAPBA) (9:1) on the surface of 3-mercaptopropyl-silylated glass plates and capillaries. The brushes were characterized with time-of-flight secondary ion mass-spectrometry (ToF SIMS), atomic force microscopy and contact angle measurements. Fructose caused a well-expressed drop spreading on the surface of copolymer-grafted glass, due to the strong interaction with the boronate groups. Sedimentation of murine hybridoma cells M2139 or human myeloid leukemia cells KG1 onto the DMAA-NAAPBA copolymer-grafted glass plates from 10 mM phosphate buffer solution (pH 8.0) resulted in the cell adhesion. The adhered M2139 and KG1 cells could be quantitatively detached from the grafted plates with 0.1M fructose, which competed with cell surface carbohydrates for binding to the boronates. Evaluation of the binding strength between M2139 cells and the copolymer brush was performed by exposure of the adhered cells to a shear stress. Detachment of a fraction of 18% of the adhered M2139 cells was obtained at a shear force of 1400-2800 pN/cell generated by the running phosphate buffer (pH 8.0), whereas the remaining adhered cells (70%) could be detached with 0.1M fructose dissolved in the same buffer. Possible applications of the boronate-containing polymer brushes to affinity cell separation can be based upon the facile recovery of the attached cells.

Original languageEnglish
Pages (from-to)213-225
Number of pages13
JournalJournal of Biomedical Materials Research - Part A
Volume88
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

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Brushes
Fructose
Polymers
Cells
Buffers
Copolymers
Glass
Phosphates
Cell adhesion
Carbohydrates
Angle measurement
Secondary ion mass spectrometry
Free radicals
Sedimentation
Copolymerization
Contact angle
Free Radicals
Shear stress
Atomic force microscopy
Recovery

Keywords

  • Affinity
  • Boronic acid
  • Cell adhesion
  • Silane
  • Surface grafting

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Ivanov, A. E., Eccles, J., Panahi, H. A., Kumar, A., Kuzimenkova, M. V., Nilsson, L., ... Mattiasson, B. (2009). Boronate-containing polymer brushes: Characterization, interaction with saccharides and mammalian cancer cells. Journal of Biomedical Materials Research - Part A, 88(1), 213-225. https://doi.org/10.1002/jbm.a.31880

Boronate-containing polymer brushes : Characterization, interaction with saccharides and mammalian cancer cells. / Ivanov, Alexander E.; Eccles, John; Panahi, Homayon Ahmad; Kumar, Ashok; Kuzimenkova, Marina V.; Nilsson, Lars; Bergenståhl, Björn; Long, Nick; Phillips, Gary J.; Mikhalovsky, Sergey V.; Galaev, Igor Yu; Mattiasson, Bo.

In: Journal of Biomedical Materials Research - Part A, Vol. 88, No. 1, 01.2009, p. 213-225.

Research output: Contribution to journalArticle

Ivanov, AE, Eccles, J, Panahi, HA, Kumar, A, Kuzimenkova, MV, Nilsson, L, Bergenståhl, B, Long, N, Phillips, GJ, Mikhalovsky, SV, Galaev, IY & Mattiasson, B 2009, 'Boronate-containing polymer brushes: Characterization, interaction with saccharides and mammalian cancer cells', Journal of Biomedical Materials Research - Part A, vol. 88, no. 1, pp. 213-225. https://doi.org/10.1002/jbm.a.31880
Ivanov, Alexander E. ; Eccles, John ; Panahi, Homayon Ahmad ; Kumar, Ashok ; Kuzimenkova, Marina V. ; Nilsson, Lars ; Bergenståhl, Björn ; Long, Nick ; Phillips, Gary J. ; Mikhalovsky, Sergey V. ; Galaev, Igor Yu ; Mattiasson, Bo. / Boronate-containing polymer brushes : Characterization, interaction with saccharides and mammalian cancer cells. In: Journal of Biomedical Materials Research - Part A. 2009 ; Vol. 88, No. 1. pp. 213-225.
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