Cathepsin G is differentially expressed in primary human antigen-presenting cells

Christina Stoeckle, Vinod Sommandas, Eleni Adamopoulou, Kurt Belisle, Stephan Schiekofer, Arthur Melms, Ekkehard Weber, Christoph Driessen, Bernhard O Boehm, Eva Tolosa, Timo Burster

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cathepsins are required for the processing of antigens in order to make them suitable for loading on major histocompatibility complex (MHC) class II molecules, for subsequent presentation to CD4(+) T cells. It was shown that antigen processing in monocyte-derived dendritic cells (DC), a commonly used DC model, is different from that of primary human DC. Here, we report that the two subsets of human myeloid DC (mDC) and plasmacytoid DC (pDC) differ in their cathepsin distribution. The serine protease cathepsin G (CatG) was detected in mDC1, mDC2, pDC, cortical thymic epithelial cells (cTEC) and high levels of CatG were determined in pDC. To address the role of CatG in the processing and presentation of a Multiple Sclerosis-associated autoantigen myelin basic protein (MBP), we used a non-CatG expressing fibroblast cell line and fibroblasts, which were preloaded with purified CatG. We find that preloading fibroblasts with CatG results in a decrease of MBP84-98-specific T cell proliferation, when compared to control cells. Our data suggest a different processing signature in primary human antigen-presenting cells and CatG may be of functional importance.

Original languageEnglish
Pages (from-to)41-5
Number of pages5
JournalCellular Immunology
Volume255
Issue number1-2
DOIs
Publication statusPublished - 2009

Keywords

  • Antigen Presentation
  • Antigen-Presenting Cells
  • Aspartic Acid Endopeptidases
  • Autoantigens
  • Cathepsin G
  • Cathepsins
  • Cell Line
  • Cysteine Endopeptidases
  • Humans
  • Male
  • Multiple Sclerosis
  • Myelin Basic Protein
  • Serine Endopeptidases
  • Journal Article
  • Research Support, Non-U.S. Gov't

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    Stoeckle, C., Sommandas, V., Adamopoulou, E., Belisle, K., Schiekofer, S., Melms, A., Weber, E., Driessen, C., Boehm, B. O., Tolosa, E., & Burster, T. (2009). Cathepsin G is differentially expressed in primary human antigen-presenting cells. Cellular Immunology, 255(1-2), 41-5. https://doi.org/10.1016/j.cellimm.2008.10.001