Cell Encapsulation Within Alginate Microcapsules: Immunological Challenges and Outlook

Assem Ashimova, Sergey Yegorov, Baurzhan Negmetzhanov, Gonzalo Hortelano

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

Cell encapsulation is a bioengineering technology that provides live allogeneic or xenogeneic cells packaged in a semipermeable immune-isolating membrane for therapeutic applications. The concept of cell encapsulation was first proposed almost nine decades ago, however, and despite its potential, the technology has yet to deliver its promise. The few clinical trials based on cell encapsulation have not led to any licensed therapies. Progress in the field has been slow, in part due to the complexity of the technology, but also because of the difficulties encountered when trying to prevent the immune responses generated by the various microcapsule components, namely the polymer, the encapsulated cells, the therapeutic transgenes and the DNA vectors used to genetically engineer encapsulated cells. While the immune responses induced by polymers such as alginate can be minimized using highly purified materials, the need to cope with the immunogenicity of encapsulated cells is increasingly seen as key in preventing the immune rejection of microcapsules. The encapsulated cells are recognized by the host immune cells through a bidirectional exchange of immune mediators, which induce both the adaptive and innate immune responses against the engrafted capsules. The potential strategies to cope with the immunogenicity of encapsulated cells include the selective diffusion restriction of immune mediators through capsule pores and more recently inclusion in microcapsules of immune modulators such as CXCL12. Combining these strategies with the use of well-characterized cell lines harboring the immunomodulatory properties of stem cells should encourage the incorporation of cell encapsulation technology in state-of-the-art drug development.

Original languageEnglish
Article number380
JournalFrontiers in Bioengineering and Biotechnology
Volume7
DOIs
Publication statusPublished - Dec 3 2019

Keywords

  • alginate
  • cell encapsulation
  • cytokines
  • damage-associated molecular patterns
  • immune response
  • microcapsule
  • therapeutic delivery

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Cell Encapsulation Within Alginate Microcapsules: Immunological Challenges and Outlook'. Together they form a unique fingerprint.

  • Cite this