Centrioles in the cell cycle. I. Epithelial cells

I. A. Vorobjev, S. Chentsov Yu.

Research output: Contribution to journalArticle

247 Citations (Scopus)

Abstract

A study was made of the structure of the centrosome in the cell cycle in a nonsynchronous culture of pig kidney embryo (PE) cells. In the spindle pole of the metaphase cell there are two mutually perpendicular centrioles (mother and daughter) which differ in their ultrastructure. An electron-dense halo, which surrounds only the mother centriole and is the site where spindle microtubules converge, disappears at the end of telophase. In metaphase and anaphase, the mother centriole is situated perpendicular to the spindle axis. At the beginning of the G1 period, pericentriolar satellites are formed on the mother centriole with microtubules attached to them; the two centrioles diverge. The structures of the two centrioles differ throughout interphase; the mother centriole has appendages, the daughter does not. Replication of the centrioles occurs approximately in the middle of the S period. The structure of the procentrioles differs sharply from that of the mature centriole. Elongation of procentrioles is completed in prometaphase, and their structure undergoes a number of successive changes. In the G2 period, pericentriolar satellites disappear and some time later a fibrillar halo is formed on both mother centrioles, i.e., spindle poles begin to form. In the cells that have left the mitotic cycle (G0 period), replication of centrioles does not take place; in many cells, a cilium is formed on the mother centriole. In a small number of cells a cilium is formed in the S and G2 periods, but unlike the cilium in the G0 period it does not reach the surface of the cell. In all cases, it locates on the centriole with appendages. At the beginning of the G1 period, during the G2 period, and in nonciliated cells in the G0 period, one of the centrioles is situated perpendicular to the substrate. On the whole, it takes a mature centriole a cycle and a half to form in PE cells.

Original languageEnglish
Pages (from-to)938-949
Number of pages12
JournalJournal of Cell Biology
Volume93
Issue number3
Publication statusPublished - 1982
Externally publishedYes

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Centrioles
Cell Cycle
Epithelial Cells
Cilia
Spindle Poles
Metaphase
S Phase
Microtubules
Swine
Embryonic Structures
Prometaphase
Telophase
Kidney
Centrosome
Anaphase
Interphase

ASJC Scopus subject areas

  • Cell Biology

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Centrioles in the cell cycle. I. Epithelial cells. / Vorobjev, I. A.; Chentsov Yu., S.

In: Journal of Cell Biology, Vol. 93, No. 3, 1982, p. 938-949.

Research output: Contribution to journalArticle

Vorobjev, IA & Chentsov Yu., S 1982, 'Centrioles in the cell cycle. I. Epithelial cells', Journal of Cell Biology, vol. 93, no. 3, pp. 938-949.
Vorobjev, I. A. ; Chentsov Yu., S. / Centrioles in the cell cycle. I. Epithelial cells. In: Journal of Cell Biology. 1982 ; Vol. 93, No. 3. pp. 938-949.
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