Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water

Lyuba I. Mikhalovska, Vlad M. Gun'ko, Vlad V. Turov, Vlad I. Zarko, Stuart L. James, Pankaj Vadgama, Paul E. Tomlins, Sergey Victorovich Mikhalovsky

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The nanoporous structure of collagen-glycosaminoglycan (CG) hydrogels was studied using 1H NMR spectroscopy and thermally stimulated depolarisation (TSD) current with layer-by-layer freezing-out of bulk and interfacial water. The depression of the freezing point of water is related to the size of the nanopore, to which it is confined. Changes in the Gibbs free energy of the unfrozen interfacial water are related to the amount of bound water in the hydrogel matrix and to the re-arrangement of the 3D network structure of the biopolymer. Analysis of the thermodynamic properties of bulk and interfacial water using the layer-by-layer freezing-out technique combined with NMR and TSDC provides valuable information about the structural features of CG hydrogels that can be used for characterisation of different types of hydrogels and soft tissue scaffolds, artificial skin substitutes and other biomaterials.

Original languageEnglish
Pages (from-to)3599-3607
Number of pages9
JournalBiomaterials
Volume27
Issue number19
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Fingerprint

Hydrogels
Glycosaminoglycans
Collagen
Freezing
Water
Artificial Skin
Nanopores
Tissue Scaffolds
Biopolymers
Hydrogel
Depolarization
Biocompatible Materials
Gibbs free energy
Thermodynamics
Biomaterials
Nuclear magnetic resonance spectroscopy
Skin
Magnetic Resonance Spectroscopy
Thermodynamic properties
Nuclear magnetic resonance

Keywords

  • Collagen
  • Hydrogel
  • Nanopore structure
  • NMR
  • Porosity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Mikhalovska, L. I., Gun'ko, V. M., Turov, V. V., Zarko, V. I., James, S. L., Vadgama, P., ... Mikhalovsky, S. V. (2006). Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water. Biomaterials, 27(19), 3599-3607. https://doi.org/10.1016/j.biomaterials.2006.02.010

Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water. / Mikhalovska, Lyuba I.; Gun'ko, Vlad M.; Turov, Vlad V.; Zarko, Vlad I.; James, Stuart L.; Vadgama, Pankaj; Tomlins, Paul E.; Mikhalovsky, Sergey Victorovich.

In: Biomaterials, Vol. 27, No. 19, 07.2006, p. 3599-3607.

Research output: Contribution to journalArticle

Mikhalovska, LI, Gun'ko, VM, Turov, VV, Zarko, VI, James, SL, Vadgama, P, Tomlins, PE & Mikhalovsky, SV 2006, 'Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water', Biomaterials, vol. 27, no. 19, pp. 3599-3607. https://doi.org/10.1016/j.biomaterials.2006.02.010
Mikhalovska, Lyuba I. ; Gun'ko, Vlad M. ; Turov, Vlad V. ; Zarko, Vlad I. ; James, Stuart L. ; Vadgama, Pankaj ; Tomlins, Paul E. ; Mikhalovsky, Sergey Victorovich. / Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water. In: Biomaterials. 2006 ; Vol. 27, No. 19. pp. 3599-3607.
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