Characteristics of interfacial water affected by proteins adsorbed on activated carbon

T. A. Alexeeva, N. I. Lebovka, V. M. Gun'ko, V. V. Strashko, S. V. Mikhalovsky

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The influence of proteins (bovine serum albumin, BSA, and mouse γ-globulin, IgG) physically adsorbed or covalently attached via coupling with N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimide methyl-p- toluenesulfonate, CMC, to the surface of activated carbon SCN (spherical carbon with nitrogen) on the mobility of interfacial water molecules was studied by means of 1H NMR spectroscopy with freezing-out of bulk water at 180<T<273 K. Relaxation processes in the interfacial non-freezing water were investigated measuring transverse time t2 of proton relaxation dependence on the presence of proteins and CMC. The distribution function of activation free energy of relaxation (with a maximum at 20-22 kJ/mol) was calculated for the protein-water-carbon systems using a regularization procedure and the relationships between t2 and the amounts of the interfacial water unfrozen at T<250 K assuming the Arrhenius-type dependence for t 2-1 on temperature. The state of unfrozen water in pores of SCN shows that the low temperature relaxation processes occur in narrow pores with half-width X<1.5 nm.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalJournal of Colloid and Interface Science
Volume278
Issue number2
DOIs
Publication statusPublished - Oct 15 2004

Keywords

  • Activated carbon
  • H NMR spectroscopy
  • Microporosity
  • Nonfreezable interfacial water
  • Protein chemisorption
  • Protein immobilization
  • Surface properties

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

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  • Cite this

    Alexeeva, T. A., Lebovka, N. I., Gun'ko, V. M., Strashko, V. V., & Mikhalovsky, S. V. (2004). Characteristics of interfacial water affected by proteins adsorbed on activated carbon. Journal of Colloid and Interface Science, 278(2), 333-341. https://doi.org/10.1016/j.jcis.2004.06.030